MGMT Antibody (E-6): sc-166491
- MGMT Antibody (E-6) is a mouse monoclonal IgG3 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 9-24 at the N-terminus of MGMT of human origin
- recommended for detection of MGMT of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- See MGMT (E-1): sc-166528 for MGMT antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MGMT Antibody (E-6). This work is in progress.
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SEE ALSO...
MGMT Antibody (E-6) is a mouse monoclonal IgG3 kappa light chain antibody specific for an epitope mapping between amino acids 9-24 at the N-terminus of human MGMT, showing reactivity with MGMT from mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MGMT, also known as O6-methylguanine-DNA methyltransferase or O-6-alkylguanine DNA alkyltransferase (AGT), is a crucial DNA repair enzyme that removes mutagenic and cytotoxic alkyl groups from the O6 position of guanine, thereby protecting the genome from mutations caused by alkylating agents. Post-translational modifications of MGMT, such as ubiquitination, play a significant role in regulating activity and stability. Ubiquitination leads to proteasomal degradation of MGMT, which is important for controlling cellular levels and modulating DNA repair response. MGMT regulation through these modifications is vital because MGMT overexpression can confer resistance to alkylating chemotherapeutic drugs, impacting cancer treatment efficacy. MGMT expression is transcriptionally activated in response to DNA damage, but this induction requires functional p53, a tumor suppressor protein involved in DNA repair and apoptosis. Studies have shown that anti-MGMT antibody (E-6) detects MGMT induction by ionizing radiation only in cells expressing wild-type p53, indicating a regulatory relationship between MGMT and p53. Conversely, high MGMT activity has been associated with mutant p53 in certain cancer cell lines, suggesting a complex interplay between these proteins. Understanding post-translational modifications and regulation of MGMT is essential in cancer research and therapeutic development, making MGMT (E-6) monoclonal antibody a valuable tool for researchers investigating DNA repair mechanisms and p53-related pathways.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MGMT Antibody (E-6) | sc-166491 | 200 µg/ml | $322.00 | |||
MGMT (E-6) Neutralizing Peptide | sc-166491 P | 100 µg/0.5 ml | $69.00 |