MGAT1 CRISPR/Cas9 KO Plasmid (h): sc-414091
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MGAT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MGAT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MGAT1 Antibody (H-6): sc-376079
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MGAT1 CRISPR/Cas9 KO Plasmid (h) | sc-414091 | 20 µg | $397.00 |
Overview
Human MOGAT1 encodes monoacylglycerol O-acyltransferase 1 (MGAT1), an endoplasmic reticulum membrane enzyme that catalyzes the acylation of monoacylglycerol to form diacylglycerol, a key intermediate in triacylglycerol biosynthesis. By controlling the balance of monoacylglycerol, diacylglycerol, and downstream lipid pools, MGAT1 contributes to lipid droplet formation and broader cellular lipid homeostasis. Altered MGAT1 activity has been linked to metabolic phenotypes, including dysregulated hepatic lipid accumulation and insulin signaling, and is studied in the context of obesity-associated steatosis and related inflammatory stress pathways. These functions make MOGAT1 a relevant target for investigating lipid metabolic rewiring and organ-specific regulation of neutral lipid storage.
MGAT1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MOGAT1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MOGAT1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MOGAT1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MGAT1 protein expression.
This CRISPR knockout system enables efficient generation of MOGAT1-deficient cell models for investigation of MGAT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MOGAT1 exon(s) critical for MGAT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MOGAT1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MGAT1 CRISPR/Cas9 KO Plasmid (h) and MGAT1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MOGAT1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MGAT1 HDR Plasmid (h) and MGAT1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MOGAT1 homology arms to support homology-directed repair at defined MOGAT1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.