MGAT1 Antibody (H-6): sc-376079

MGAT1 Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects MGAT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MGAT1 (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MGAT1 protein, also known as monoacylglycerol O-acyltransferase 1, plays a crucial role in lipid metabolism by catalyzing the conversion of diacylglycerol to triacylglycerol, a key process in lipoprotein synthesis that transport dietary fats throughout the body. This function is particularly important for intestinal absorption of dietary fats, as triacylglycerol resynthesis is essential for assembling lipoproteins that facilitate absorbed fat transport to various tissues. MGAT1 expression occurs in the stomach, kidney, liver, and adipose tissue, but remains absent in the intestine, distinguishing MGAT1 from MGAT2, which is predominantly expressed in the small intestine and plays a different role in lipid metabolism. Understanding specific expression patterns and functions of MGAT1 remains vital for elucidating contributions to metabolic processes and potential implications in metabolic disorders.

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MGAT1 Antibody (H-6) References:

1. Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members.  |  Cases, S., et al. 2001. J Biol Chem. 276: 38870-6. PMID: 11481335
2. Identification of a gene encoding MGAT1, a monoacylglycerol acyltransferase.  |  Yen, CL., et al. 2002. Proc Natl Acad Sci U S A. 99: 8512-7. PMID: 12077311
3. Identification of acyl coenzyme A:monoacylglycerol acyltransferase 3, an intestinal specific enzyme implicated in dietary fat absorption.  |  Cheng, D., et al. 2003. J Biol Chem. 278: 13611-4. PMID: 12618427
4. MGAT2, a monoacylglycerol acyltransferase expressed in the small intestine.  |  Yen, CL. and Farese, RV. 2003. J Biol Chem. 278: 18532-7. PMID: 12621063
5. A predominant role of acyl-CoA:monoacylglycerol acyltransferase-2 in dietary fat absorption implicated by tissue distribution, subcellular localization, and up-regulation by high fat diet.  |  Cao, J., et al. 2004. J Biol Chem. 279: 18878-86. PMID: 14966132
6. Catalytic properties of MGAT3, a putative triacylgycerol synthase.  |  Cao, J., et al. 2007. J Lipid Res. 48: 583-91. PMID: 17170429
7. MGAT1 and Complex N-Glycans Regulate ERK Signaling During Spermatogenesis.  |  Biswas, B., et al. 2018. Sci Rep. 8: 2022. PMID: 29386567
8. LINC00173 promotes Wilms' tumor progression through MGAT1-mediated MUC3A N-glycosylation.  |  Zhu, Q., et al. 2022. Cell Cycle. 21: 1795-1810. PMID: 35491865
9. Role of glycosylation-related gene MGAT1 in pancreatic ductal adenocarcinoma.  |  Jiang, L., et al. 2024. Front Immunol. 15: 1438935. PMID: 39156890
10. Lactate supports Treg function and immune balance via MGAT1 effects on N-glycosylation in the mitochondria.  |  Zhou, J., et al. 2024. J Clin Invest. 134: PMID: 39264847
11. Solubilization and partial purification of neonatally expressed rat hepatic microsomal monoacylglycerol acyltransferase.  |  Bhat, BG., et al. 1993. Arch Biochem Biophys. 300: 663-9. PMID: 8382031
12. Stereospecificity of monoacylglycerol and diacylglycerol acyltransferases from rat intestine as determined by chiral phase high-performance liquid chromatography.  |  Lehner, R., et al. 1993. Lipids. 28: 29-34. PMID: 8446008

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MGAT1 Antibody (H-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-MGAT1 Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MGAT1 Antibody (H-6) Specifications

Alternate Product Name: MGAT1

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 119-208

Epitope Target: MGAT1

Epitope Species: mouse

Product Reactivity/Detection: MGAT1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for MGAT1 Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin