Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MFF CRISPR/Cas9 KO Plasmid (h): sc-402756
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MFF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MFF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MFF Antibody (B-2): sc-398617
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MFF CRISPR/Cas9 KO Plasmid (h) | sc-402756 | 20 µg | $397.00 |
Overview
Human MFF (mitochondrial fission factor) is an outer mitochondrial membrane adaptor that recruits the dynamin-related GTPase DRP1 to promote mitochondrial and peroxisomal division, shaping organelle morphology and distribution. Through control of fission–fusion balance, MFF influences mitochondrial network architecture, mitophagy, apoptosis sensitivity, and cellular bioenergetics, with downstream effects on oxidative phosphorylation, ROS handling, and stress signaling. Perturbation of MFF-dependent fission is linked to altered mitochondrial dynamics and has been associated with neurodevelopmental and neurodegenerative phenotypes, as well as broader metabolic dysfunction where organelle quality control is disrupted. As a core node in mitochondrial dynamics pathways, MFF is frequently studied in contexts of organelle homeostasis, innate immune signaling, and cell fate decisions.
MFF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MFF gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MFF together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MFF open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MFF protein expression.
This CRISPR knockout system enables efficient generation of MFF-deficient cell models for investigation of MFF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MFF exon(s) critical for MFF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MFF genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MFF CRISPR/Cas9 KO Plasmid (h) and MFF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MFF locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MFF HDR Plasmid (h) and MFF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MFF homology arms to support homology-directed repair at defined MFF target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.