Met/Leu-enkephalin Antibody (NOC1/35): sc-47705

Met/Leu-enkephalin Antibody (NOC1/35) is a mouse monoclonal IgG1 kappa light chain antibody that detects Met/Leu-enkephalin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Met/Leu-enkephalin antibody (NOC1/35) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Met/Leu-enkephalin, derived from the proenkephalin precursor, plays a critical role in modulating pain and stress responses in the body. Met/Leu-enkephalin (NOC1/35) monoclonal antibody is primarily located in the central nervous system and is involved in the regulation of nociception, which is the sensory perception of pain. This localization enables Met/Leu-enkephalin to interact with opioid receptors in the brain, leading to analgesic effects and influencing emotional responses to stress. The presence of Met/Leu-enkephalin in peripheral tissues suggests potential roles in various physiological processes beyond pain modulation, including immune response and neuroprotection. Anti-Met/Leu-enkephalin antibody (NOC1/35) helps researchers gain valuable insights into the functional dynamics of this important neuropeptide and its implications in pain management and stress-related disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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Met/Leu-enkephalin Antibody (NOC1/35) References:

1. A new electrochemical HPLC method for analysis of enkephalins and endomorphins.  |  Lisi, TL. and Sluka, KA. 2006. J Neurosci Methods. 150: 74-9. PMID: 16051372
2. Ischemic preconditioning increases the bioavailability of cardiac enkephalins.  |  Younès, A., et al. 2005. Am J Physiol Heart Circ Physiol. 289: H1652-61. PMID: 16162869
3. Nucleotide and amino acid sequence of lymphocyte-derived corticotropin: endotoxin induction of a truncated peptide.  |  Smith, EM., et al. 1990. Proc Natl Acad Sci U S A. 87: 1057-60. PMID: 1689057
4. Plasma met-enkephalin, beta-endorphin and leu-enkephalin levels in human hepatic encephalopathy.  |  Kamel, L., et al. 2007. East Mediterr Health J. 13: 257-65. PMID: 17684846
5. Corticotropin and beta-endorphin: construction and analysis of recombinant DNA complementary to mRNA for the common precursor.  |  Roberts, JL., et al. 1979. Proc Natl Acad Sci U S A. 76: 2153-7. PMID: 221916
6. Expression of mouse proopiomelanocortin in an insulinoma cell line. Requirements for beta-endorphin processing.  |  Thorne, BA., et al. 1989. J Biol Chem. 264: 3545-52. PMID: 2536749
7. Detection of Met-enkephalin and Leu-enkephalin in the posterior pituitary of the holostean fish, Amia calva.  |  Dores, RM., et al. 1989. Peptides. 10: 951-6. PMID: 2608557
8. On the origin of Leu-enkephalin and Met-enkephalin in the rat neurohypophysis.  |  Zamir, N. 1985. Endocrinology. 117: 1687-92. PMID: 2863131
9. Isolation and characterization of the mouse corticotropin-beta-lipotropin precursor gene and a related pseudogene.  |  Notake, M., et al. 1983. FEBS Lett. 156: 67-71. PMID: 6303853
10. Immunocytochemical and ultrastructural differentiation between Met-enkephalin-, Leu-enkephalin-, and Met/Leu-enkephalin-immunoreactive neurons of feline gut.  |  Larsson, LI. and Stengaard-Pendersen, K. 1982. J Neurosci. 2: 861-78. PMID: 7047687
11. Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA.  |  Comb, M., et al. 1982. Nature. 295: 663-6. PMID: 7057924
12. Met- and leu-enkephalin immuno- and bio-reactivity in human stomach and pancreas.  |  Feurle, GE., et al. 1982. Life Sci. 31: 2961-9. PMID: 7162359

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Met/Leu-enkephalin Antibody (NOC1/35) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Met/Leu-enkephalin Antibody (NOC1/35) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Met/Leu-enkephalin Antibody (NOC1/35) Specifications

Alternate Product Name: Met enkephalin

Product Clone Name: NOC1/35

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Met/Leu-enkephalin

Epitope Species: human

Product Reactivity/Detection: Proenkaphalin A and the processed active peptides Met-enkephalin and Leu-enkephalin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Met/Leu-enkephalin Antibody (NOC1/35): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin