Anti-MESP1 Antibody (JH.12) recommended for detection of MESP1 of mouse, rat and human origin by WB and IP

Anti-MESP1 Antibody (JH.12): sc-130461

Anti-MESP1 Antibody (JH.12) is rated 4.0 out of 5 by 3.
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Datasheets

    • Anti-MESP1 Antibody (JH.12) is a mouse monoclonal IgG2a κ MESP1 antibody, cited in 4 publications, provided at 100 µg/ml
    • raised against a partial recombinant protein mapping within amino acids 1-63 of MESP1 of human origin
    • recommended for detection of MESP1 of mouse, rat and human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MESP1 (JH.12): sc-130461.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for MESP1 Antibody (JH.12) for WB applications. These reagents are now offered in bundles with MESP1 Antibody (JH.12) (see ordering information below).
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I am using MESP1 (JH.12): sc-130461 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Not too bad for IHC We are continuing to work on the IHC with this antibody, but there first couple stains using buffer/heat antigen retrieval and amplification of the signal looks promising.
Date published: 2017-09-20
Rated 5 out of 5 by from excellent I have used this antibody sample in differentiation of embryonic stem cells,result shows that the antibody is very specific。
Date published: 2016-11-16
Rated 4 out of 5 by from ICC publishable data with human human pluripotent ICC publishable data with human human pluripotent stem cells (hPSCs) - SCBT Publication Review
Date published: 2014-09-02
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