MESP1 Antibody (JH.12): sc-130461

MESP1 Antibody (JH.12) is a mouse monoclonal IgG2a kappa light chain antibody that detects MESP1 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-MESP1 antibody (JH.12) is available as a non-conjugated format. MESP1, or mesoderm posterior 1 homolog, is a 268 amino acid protein characterized by a basic helix-loop-helix (bHLH) domain, which is crucial for protein dimerization and binding to E-box sequences in DNA. MESP1 is primarily localized in the nucleus, where MESP1 functions as a transcription factor, playing a vital role in the epithelialization and development of cardiac and somitic mesoderm. MESP1 expression peaks during gastrulation and somitogenesis, which are critical stages in embryonic development. MESP1 is essential for the formation of single heart tubes during cardiac maturation, and early detection can serve as an indicator of cardiac precursor cell formation in developing embryos. Additionally, MESP1 is involved in the rostrocaudal patterning of somites, influencing specific Notch signaling pathways that are crucial for proper embryonic development.

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MESP1 Antibody (JH.12) References:

1. MesP1 is expressed in the heart precursor cells and required for the formation of a single heart tube.  |  Saga, Y., et al. 1999. Development. 126: 3437-47. PMID: 10393122
2. MesP1 and MesP2 are essential for the development of cardiac mesoderm.  |  Kitajima, S., et al. 2000. Development. 127: 3215-26. PMID: 10887078
3. Mesp1 expression is the earliest sign of cardiovascular development.  |  Saga, Y., et al. 2000. Trends Cardiovasc Med. 10: 345-52. PMID: 11369261
4. Transcriptional regulation of Mesp1 and Mesp2 genes: differential usage of enhancers during development.  |  Haraguchi, S., et al. 2001. Mech Dev. 108: 59-69. PMID: 11578861
5. Mutated MESP2 causes spondylocostal dysostosis in humans.  |  Whittock, NV., et al. 2004. Am J Hum Genet. 74: 1249-54. PMID: 15122512
6. MesP1 drives vertebrate cardiovascular differentiation through Dkk-1-mediated blockade of Wnt-signalling.  |  David, R., et al. 2008. Nat Cell Biol. 10: 338-45. PMID: 18297060
7. Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.  |  Lindsley, RC., et al. 2008. Cell Stem Cell. 3: 55-68. PMID: 18593559
8. Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification.  |  Bondue, A., et al. 2008. Cell Stem Cell. 3: 69-84. PMID: 18593560
9. Mesp1 controls the chromatin and enhancer landscapes essential for spatiotemporal patterning of early cardiovascular progenitors.  |  Lin, X., et al. 2022. Nat Cell Biol. 24: 1114-1128. PMID: 35817961
10. Essential role of MESP1-RING1A complex in cardiac differentiation.  |  Liang, Q., et al. 2022. Dev Cell. 57: 2533-2549.e7. PMID: 36413948
11. A Mesp1-dependent developmental breakpoint in transcriptional and epigenomic specification of early cardiac precursors.  |  Krup, AL., et al. 2023. Development. 150: PMID: 36994838
12. MesP1: a novel basic helix-loop-helix protein expressed in the nascent mesodermal cells during mouse gastrulation.  |  Saga, Y., et al. 1996. Development. 122: 2769-78. PMID: 8787751

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MESP1 Antibody (JH.12) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-MESP1 Antibody (JH.12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MESP1 Antibody (JH.12) Specifications

Alternate Product Name: MESP1

Product Clone Name: JH.12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-63

Epitope Target: MESP1

Epitope Species: human

Product Reactivity/Detection: MESP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for MESP1 Antibody (JH.12): N/A