MEPE Antibody (C-4): sc-377035

MEPE Antibody (C-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects MEPE protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MEPE (C-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MEPE, or matrix extracellular phosphoglycoprotein, is a 525 amino acid extracellular matrix protein primarily expressed in osteocytes and the brain, playing a crucial role in regulating bone metabolism. One of the key aspects of MEPE is its RGD cell-attachment motif, which facilitates interactions with integrins, thereby influencing cell adhesion and signaling. This structural feature is vital for MEPE′s function in mediating mineralization and demineralization processes within the osteocyte microenvironment, which are essential for maintaining bone health and integrity. Additionally, MEPE is implicated in various developmental processes, including skeletogenesis, bone regeneration, and odontogenesis, highlighting MEPE′s importance in both normal physiology and potential pathophysiological conditions. Notably, MEPE is secreted in hypophosphatemic osteomalacia tumors, suggesting MEPE′s involvement in the pathophysiology of bone-related cancers. Defects in the gene encoding MEPE may lead to osteomalacia, a condition characterized by inadequate bone mineralization, further underscoring MEPE′s significance in skeletal health.

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MEPE Antibody (C-4) References:

1. MEPE, a new gene expressed in bone marrow and tumors causing osteomalacia.  |  Rowe, PS., et al. 2000. Genomics. 67: 54-68. PMID: 10945470
2. MEPE has the properties of an osteoblastic phosphatonin and minhibin.  |  Rowe, PS., et al. 2004. Bone. 34: 303-19. PMID: 14962809
3. Mepe is expressed during skeletal development and regeneration.  |  Lu, C., et al. 2004. Histochem Cell Biol. 121: 493-9. PMID: 15221418
4. MEPE expression in osteocytes during orthodontic tooth movement.  |  Gluhak-Heinrich, J., et al. 2007. Arch Oral Biol. 52: 684-90. PMID: 17270144
5. Matrix extracellular phosphoglycoprotein (MEPE) is a new bone renal hormone and vascularization modulator.  |  David, V., et al. 2009. Endocrinology. 150: 4012-23. PMID: 19520780
6. Inhibition of osteoclastogenesis by mechanically loaded osteocytes: involvement of MEPE.  |  Kulkarni, RN., et al. 2010. Calcif Tissue Int. 87: 461-8. PMID: 20725825
7. Surface immobilization of MEPE peptide onto HA/β-TCP ceramic particles enhances bone regeneration and remodeling.  |  Acharya, B., et al. 2012. J Biomed Mater Res B Appl Biomater. 100: 841-9. PMID: 22278974
8. MEPE is a novel regulator of growth plate cartilage mineralization.  |  Staines, KA., et al. 2012. Bone. 51: 418-30. PMID: 22766095
9. Variants affecting diverse domains of MEPE are associated with two distinct bone disorders, a craniofacial bone defect and otosclerosis.  |  Schrauwen, I., et al. 2019. Genet Med. 21: 1199-1208. PMID: 30287925
10. Active sites of human MEPE-ASARM regulating bone matrix mineralization.  |  Minamizaki, T., et al. 2020. Mol Cell Endocrinol. 517: 110931. PMID: 32712387

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEPE Antibody (C-4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-MEPE Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEPE Antibody (C-4) Specifications

Alternate Product Name: MEPE

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: MEPE

Epitope Species: rat

Product Reactivity/Detection: MEPE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for MEPE Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MEPE antibody is MEPE Antibody (LFMb-33): sc-73635.