MEPE Antibody (LFMb-33): sc-73635

MEPE Antibody (LFMb-33) is a mouse monoclonal IgG1 kappa light chain antibody that detects MEPE (matrix extracellular phosphoglycoprotein) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MEPE antibody (LFMb-33) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MEPE, also known as osteoblast/osteocyte factor 45 (OF45), is a 525 amino acid extracellular matrix protein predominantly expressed in osteocytes and the brain. MEPE plays a crucial role in regulating bone metabolism, particularly in mediating mineralization and demineralization processes within the osteocyte microenvironment. Notably, MEPE contains an RGD cell-attachment motif, which is essential for cell adhesion and signaling, and shares structural similarities with other RGD-containing phosphoglycoproteins such as osteopontin (OPN) and dentin sialophosphoprotein (DSP). MEPE′s ability to influence bone mineralization is vital for various developmental processes, including skeletogenesis, bone regeneration, and odontogenesis. Additionally, MEPE is secreted in hypophosphatemic osteomalacia tumors, indicating potential involvement in the pathophysiology of bone-related cancers. Genetic defects in the MEPE gene have been linked to osteomalacia, a condition characterized by inadequate bone mineralization, which can manifest as an adult form of rickets.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MEPE Antibody (LFMb-33) References:

1. MEPE/OF45, a new dentin/bone matrix protein and candidate gene for dentin diseases mapping to chromosome 4q21.  |  MacDougall, M., et al. 2002. Connect Tissue Res. 43: 320-30. PMID: 12489176
2. FGF23, PHEX, and MEPE regulation of phosphate homeostasis and skeletal mineralization.  |  Quarles, LD. 2003. Am J Physiol Endocrinol Metab. 285: E1-9. PMID: 12791601
3. Matrix extracellular phosphoglycoprotein (MEPE) is highly expressed in osteocytes in human bone.  |  Nampei, A., et al. 2004. J Bone Miner Metab. 22: 176-84. PMID: 15108058
4. Mepe is expressed during skeletal development and regeneration.  |  Lu, C., et al. 2004. Histochem Cell Biol. 121: 493-9. PMID: 15221418
5. Serum MEPE-ASARM-peptides are elevated in X-linked rickets (HYP): implications for phosphaturia and rickets.  |  Bresler, D., et al. 2004. J Endocrinol. 183: R1-9. PMID: 15590969
6. Expression of phosphoproteins and amelotin in teeth.  |  Trueb, B., et al. 2007. Int J Mol Med. 19: 49-54. PMID: 17143547
7. MEPE expression in osteocytes during orthodontic tooth movement.  |  Gluhak-Heinrich, J., et al. 2007. Arch Oral Biol. 52: 684-90. PMID: 17270144
8. Dentonin, a MEPE fragment, initiates pulp-healing response to injury.  |  Six, N., et al. 2007. J Dent Res. 86: 780-5. PMID: 17652210
9. DMP1 and MEPE expression are elevated in osteocytes after mechanical loading in vivo: theoretical role in controlling mineral quality in the perilacunar matrix.  |  Harris, SE., et al. 2007. J Musculoskelet Neuronal Interact. 7: 313-5. PMID: 18094489
10. Degradation of MEPE, DMP1, and release of SIBLING ASARM-peptides (minhibins): ASARM-peptide(s) are directly responsible for defective mineralization in HYP.  |  Martin, A., et al. 2008. Endocrinology. 149: 1757-72. PMID: 18162525
11. Radiation activated CHK1/MEPE pathway may contribute to microgravity-induced bone density loss.  |  Zhang, X., et al. 2015. Life Sci Space Res (Amst). 7: 53-6. PMID: 26553637
12. MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation.  |  Gullard, A., et al. 2016. J Histochem Cytochem. 64: 224-36. PMID: 26927967

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEPE Antibody (LFMb-33) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-MEPE Antibody (LFMb-33) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEPE Antibody (LFMb-33) Specifications

Alternate Product Name: MEPE

Product Clone Name: LFMb-33

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MEPE

Epitope Species: human

Product Reactivity/Detection: amino acids 213-231 of MEPE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for MEPE Antibody (LFMb-33): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin