MELK Antibody (2G2B7): sc-517201

MELK Antibody (2G2B7) is a mouse monoclonal IgG2a kappa light chain antibody that detects MELK protein of human origin by western blotting (WB), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-MELK antibody (2G2B7) is available as a non-conjugated format. Maternal embryonic leucine zipper kinase (KIAA0175, HPK38), commonly referred to as MELK, is a vital member of the Snf1/AMPK family of kinases, characterized by a kinase catalytic domain and a distinctive leucine zipper motif that consists of periodic repetition of leucine residues at every seventh position within the N-terminal catalytic domain. This structural motif is significant as leucine zippers are often found in various DNA-binding proteins, suggesting involvement in crucial protein-DNA and protein-protein interactions. MELK plays a key role in signal transduction events during egg and early embryo stages of development. Mouse and human MELK proteins share 95% sequence identity in the kinase domain, demonstrating high conservation. Northern blot analysis has shown that MELK expression is specifically restricted to spermatogonia in the testis and oocytes in the ovary, highlighting essential functions in reproductive biology.

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MELK Antibody (2G2B7) References:

1. Phosphorylation of a novel zinc-finger-like protein, ZPR9, by murine protein serine/threonine kinase 38 (MPK38).  |  Seong, HA., et al. 2002. Biochem J. 361: 597-604. PMID: 11802789
2. Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1.  |  Vulsteke, V., et al. 2004. J Biol Chem. 279: 8642-7. PMID: 14699119
3. Maternal embryonic leucine zipper kinase (MELK) regulates multipotent neural progenitor proliferation.  |  Nakano, I., et al. 2005. J Cell Biol. 170: 413-27. PMID: 16061694
4. Substrate specificity and activity regulation of protein kinase MELK.  |  Beullens, M., et al. 2005. J Biol Chem. 280: 40003-11. PMID: 16216881
5. M-phase MELK activity is regulated by MPF and MAPK.  |  Badouel, C., et al. 2006. Cell Cycle. 5: 883-9. PMID: 16628004
6. The C. elegans MELK ortholog PIG-1 regulates cell size asymmetry and daughter cell fate in asymmetric neuroblast divisions.  |  Cordes, S., et al. 2006. Development. 133: 2747-56. PMID: 16774992
7. Maternal Embryonic Leucine Zipper Kinase (MELK), a Potential Therapeutic Target for Neuroblastoma.  |  Chlenski, A., et al. 2019. Mol Cancer Ther. 18: 507-516. PMID: 30674566
8. Maternal embryonic leucine zipper kinase (MELK) optimally regulates the HIV-1 uncoating process.  |  Nishiyama, T., et al. 2022. J Theor Biol. 545: 111152. PMID: 35545145
9. Maternal Embryonic Leucine Zipper Kinase is Associated with Metastasis in Triple-negative Breast Cancer.  |  Xie, X., et al. 2023. Cancer Res Commun. 3: 1078-1092. PMID: 37377604
10. MELK promotes HCC carcinogenesis through modulating cuproptosis-related gene DLAT-mediated mitochondrial function.  |  Li, Z., et al. 2023. Cell Death Dis. 14: 733. PMID: 37949877
11. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1.  |  Nagase, T., et al. 1996. DNA Res. 3: 17-24. PMID: 8724849
12. New member of the Snf1/AMPK kinase family, Melk, is expressed in the mouse egg and preimplantation embryo.  |  Heyer, BS., et al. 1997. Mol Reprod Dev. 47: 148-56. PMID: 9136115