Mel-CAM Antibody (A-9): sc-374556

Mel-CAM Antibody (A-9) is a mouse monoclonal IgG1 antibody that detects Mel-CAM in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Mel-CAM, also known as MCAM, MUC18, or CD146, is a transmembrane glycoprotein encoded by the human Mel-CAM gene, which plays a crucial role in cell adhesion and is a member of the immunoglobulin superfamily. The protein consists of 603 amino acids, featuring a signal peptide, five immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail, which are essential for function. Mel-CAM is predominantly expressed in advanced primary and metastatic melanomas, as well as in neuroectodermal lineage cell lines, but is absent in normal melanocytes. Notably, Mel-CAM is present in approximately 80% of advanced primary human melanomas and is closely associated with metastatic disease progression. Mel-CAM activation triggers an outside-in signaling pathway involving protein tyrosine kinases such as Fyn, focal adhesion kinase (FAK), and paxillin, which are vital for regulating actin cytoskeleton rearrangement dynamics. This process is not only important for tumor progression but also plays a significant role in maintaining thymic architecture and function, highlighting Mel-CAM′s relevance in both cancer biology and immune system development.

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Mel-CAM Antibody (A-9) References:

1. Expression of CD146 adhesion molecules (MUC18 or MCAM) in the thymic microenvironment.  |  Seftalioğlu, A. and Karakoç, L. 2000. Acta Histochem. 102: 69-83. PMID: 10726166
2. Identification of the elements regulating the expression of the cell adhesion molecule MCAM/MUC18. Loss of AP-2 is not required for MCAM expression in melanoma cell lines.  |  Mintz-Weber, CS. and Johnson, JP. 2000. J Biol Chem. 275: 34672-80. PMID: 10938273
3. M-CAM expression as marker of poor prognosis in epithelial ovarian cancer.  |  Aldovini, D., et al. 2006. Int J Cancer. 119: 1920-6. PMID: 16804906
4. Improved Circulating Tumor Cell Detection by a Combined EpCAM and MCAM CellSearch Enrichment Approach in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy.  |  Onstenk, W., et al. 2015. Mol Cancer Ther. 14: 821-7. PMID: 25552367
5. The membrane protein melanoma cell adhesion molecule (MCAM) is a novel tumor marker that stimulates tumorigenesis in hepatocellular carcinoma.  |  Wang, J., et al. 2015. Oncogene. 34: 5781-95. PMID: 25728681
6. MCAM and LAMA4 Are Highly Enriched in Tumor Blood Vessels of Renal Cell Carcinoma and Predict Patient Outcome.  |  Wragg, JW., et al. 2016. Cancer Res. 76: 2314-26. PMID: 26921326
7. CD146 (MCAM) in human cs-DLK1-/cs-CD34+ adipose stromal/progenitor cells.  |  Hörl, S., et al. 2017. Stem Cell Res. 22: 1-12. PMID: 28549249
8. MCAM Mediates Chemoresistance in Small-Cell Lung Cancer via the PI3K/AKT/SOX2 Signaling Pathway.  |  Tripathi, SC., et al. 2017. Cancer Res. 77: 4414-4425. PMID: 28646020
9. MRTFB suppresses colorectal cancer development through regulating SPDL1 and MCAM.  |  Kodama, T., et al. 2019. Proc Natl Acad Sci U S A. 116: 23625-23635. PMID: 31690663
10. Pro- and anti-tumour activities of CD146/MCAM in breast cancer result from its heterogeneous expression and association with epithelial to mesenchymal transition.  |  Mannion, AJ., et al. 2023. Front Cell Dev Biol. 11: 1129015. PMID: 37138793

The preferred Mel-CAM antibody is Mel-CAM Antibody (P1H12): sc-18837.