Mel-18 Antibody (H-1): sc-515329

Mel-18 Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mel-18 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Mel-18 antibody (H-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mel-18 protein plays a crucial role in gene regulation and is part of the Polycomb group of proteins, which are essential for maintaining the repression of homeotic genes during embryonic development. Specifically, Mel-18 functions as a transcriptional repressor that binds to DNA via a RING-finger motif, allowing interaction with enhancer elements of target genes such as Bcl-2, c-Myc, and Hox. This interaction is vital for controlling cell proliferation and survival, particularly in hematopoietic cells, and highlights Mel-18′s importance as a tumor suppressor. By negatively regulating cell cycle progression and blocking entry into the S phase, Mel-18 helps prevent uncontrolled cell growth, which is a hallmark of cancer. In contrast, Mel-18′s homolog Bmi-1 has been identified as an oncogene, illustrating the delicate balance between these proteins in cellular processes. Mel-18 (H-1) antibody in various formats serves as an invaluable tool for researchers studying the intricate roles of Polycomb group proteins in development and cancer biology.

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Mel-18 Antibody (H-1) References:

1. Mice doubly deficient for the Polycomb Group genes Mel18 and Bmi1 reveal synergy and requirement for maintenance but not initiation of Hox gene expression.  |  Akasaka, T., et al. 2001. Development. 128: 1587-97. PMID: 11290297
2. The bmi-1 and mel-18 gene products define a new family of DNA-binding proteins involved in cell proliferation and tumorigenesis.  |  Goebl, MG. 1991. Cell. 66: 623. PMID: 1823128
3. Sequence similarity between the mammalian bmi-1 proto-oncogene and the Drosophila regulatory genes Psc and Su(z)2.  |  van Lohuizen, M., et al. 1991. Nature. 353: 353-5. PMID: 1922340
4. Expression and clinicopathological significance of Mel-18 and Bmi-1 mRNA in gastric carcinoma.  |  Lu, YW., et al. 2010. J Exp Clin Cancer Res. 29: 143. PMID: 21059209
5. Expression of novel DNA-binding protein with zinc finger structure in various tumor cells.  |  Tagawa, M., et al. 1990. J Biol Chem. 265: 20021-6. PMID: 2246278
6. Loss of the polycomb protein Mel-18 enhances the epithelial-mesenchymal transition by ZEB1 and ZEB2 expression through the downregulation of miR-205 in breast cancer.  |  Lee, JY., et al. 2014. Oncogene. 33: 1325-35. PMID: 23474752
7. Co-inhibition of BMI1 and Mel18 enhances chemosensitivity of esophageal squamous cell carcinoma in vitro and in vivo.  |  Wang, J., et al. 2019. Oncol Lett. 17: 5012-5022. PMID: 31186712
8. Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new 'RING-finger' motif.  |  Ishida, A., et al. 1993. Gene. 129: 249-55. PMID: 8325509
9. mel-18, a Polycomb group-related mammalian gene, encodes a transcriptional negative regulator with tumor suppressive activity.  |  Kanno, M., et al. 1995. EMBO J. 14: 5672-8. PMID: 8521824
10. A role for mel-18, a Polycomb group-related vertebrate gene, during theanteroposterior specification of the axial skeleton.  |  Akasaka, T., et al. 1996. Development. 122: 1513-22. PMID: 8625838
11. Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex.  |  Alkema, MJ., et al. 1997. Genes Dev. 11: 226-40. PMID: 9009205
12. Mammalian Polycomb group genes are categorized as a new type of early response gene induced by B-cell receptor cross-linking.  |  Hasegawa, M., et al. 1998. Mol Immunol. 35: 559-63. PMID: 9809583

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mel-18 Antibody (H-1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Mel-18 Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mel-18 Antibody (H-1) Specifications

Alternate Product Name: Mel-18

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 230-344

Epitope Target: Mel-18

Epitope Species: human

Product Reactivity/Detection: Mel-18

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Mel-18 Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent