Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MEK Kinase 1 CRISPR/Cas9 KO Plasmid (h2): sc-401107-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MEK Kinase 1 CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MEK Kinase 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MEK kinase-1 Antibody (F-11): sc-17820
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MEK Kinase 1 CRISPR/Cas9 KO Plasmid (h2) | sc-401107-KO-2 | 20 µg | $397.00 |
Overview
MAP3K1 encodes MEK kinase 1 (MEKK1), a serine/threonine MAP kinase kinase kinase that functions as an upstream regulator of stress-activated MAPK signaling. MEKK1 integrates cues from cytokines, growth factor receptors, and cytoskeletal perturbations to drive phosphorylation cascades leading to JNK and p38 activation, thereby shaping transcriptional programs controlling apoptosis, differentiation, and inflammatory responses. The protein also interfaces with ubiquitin-dependent signaling and NF-κB-related pathways, linking receptor-proximal complexes to downstream gene expression. Dysregulation of MAP3K1 signaling has been associated with altered cell fate decisions and aberrant stress response networks implicated in oncogenic and developmental contexts.
MEK Kinase 1 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the MAP3K1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAP3K1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAP3K1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MEK Kinase 1 protein expression.
This CRISPR knockout system enables efficient generation of MAP3K1-deficient cell models for investigation of MEK Kinase 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAP3K1 exon(s) critical for MEK Kinase 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAP3K1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MEK Kinase 1 CRISPR/Cas9 KO Plasmid (h) and MEK Kinase 1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAP3K1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MEK Kinase 1 HDR Plasmid (h) and MEK Kinase 1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAP3K1 homology arms to support homology-directed repair at defined MAP3K1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.