MEK kinase-1 Antibody (F-11): sc-17820

MEK kinase-1 Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects MEK kinase-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MEK kinase-1 (F-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MEK kinase-1 plays a crucial role in mitogen-activated protein (MAP) kinase signaling pathways, which are essential for cellular responses to growth factors and other extracellular signals. MEK kinase-1 is responsible for phosphorylating and activating MAP2K1 and MAP2K4, leading to the activation of extracellular signal-regulated kinases (ERK) and c-Jun NH(2)-terminal kinase (JNK) pathways. This activation regulates various cellular processes, including proliferation, differentiation, and survival. MEK kinase-1 is involved in the NFκB signaling pathway, where MEK kinase-1 activates central protein kinases such as CHUK and IKBKB, thereby influencing inflammatory responses and cell survival. MEK kinase-1′s ability to utilize an E3 ligase through its PHD domain for targeting proteins for degradation via ubiquitination highlights MEK kinase-1′s importance in maintaining cellular homeostasis and regulating protein levels within the cell.

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MEK kinase-1 Antibody (F-11) References:

1. A novel Jun N-terminal kinase (JNK)-binding protein that enhances the activation of JNK by MEK kinase 1 and TGF-beta-activated kinase 1.  |  Koyano, S., et al. 1999. FEBS Lett. 457: 385-8. PMID: 10471813
2. Activation of MEK kinase 1 by the c-Abl protein tyrosine kinase in response to DNA damage.  |  Kharbanda, S., et al. 2000. Mol Cell Biol. 20: 4979-89. PMID: 10866655
3. MEK kinase 1 interacts with focal adhesion kinase and regulates insulin receptor substrate-1 expression.  |  Yujiri, T., et al. 2003. J Biol Chem. 278: 3846-51. PMID: 12458213
4. MEK kinase 1 mediates the antiapoptotic effect of the Bcr-Abl oncogene through NF-kappaB activation.  |  Nawata, R., et al. 2003. Oncogene. 22: 7774-80. PMID: 14586403
5. MEK kinase 1 regulates c-Jun phosphorylation in the control of corneal morphogenesis.  |  Zhang, L., et al. 2003. Mol Vis. 9: 584-93. PMID: 14627958
6. MEK kinase 1 activity is required for definitive erythropoiesis in the mouse fetal liver.  |  Bonnesen, B., et al. 2005. Blood. 106: 3396-404. PMID: 16081685
7. Potentiation of apoptosis by low dose stress stimuli in cells expressing activated MEK kinase 1.  |  Widmann, C., et al. 1997. Oncogene. 15: 2439-47. PMID: 9395240
8. MEK kinase 1, a substrate for DEVD-directed caspases, is involved in genotoxin-induced apoptosis.  |  Widmann, C., et al. 1998. Mol Cell Biol. 18: 2416-29. PMID: 9528810
9. Activation of p21-CDC42/Rac-activated kinases by CD28 signaling: p21-activated kinase (PAK) and MEK kinase 1 (MEKK1) may mediate the interplay between CD3 and CD28 signals.  |  Kaga, S., et al. 1998. J Immunol. 160: 4182-9. PMID: 9574518
10. Stress-induced Fas ligand expression in T cells is mediated through a MEK kinase 1-regulated response element in the Fas ligand promoter.  |  Faris, M., et al. 1998. Mol Cell Biol. 18: 5414-24. PMID: 9710625

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEK kinase-1 Antibody (F-11) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-MEK kinase-1 Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEK kinase-1 Antibody (F-11) Specifications

Alternate Product Name: MEK kinase-1

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: MEK kinase-1

Epitope Species: human

Product Reactivity/Detection: MEK kinase-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for MEK kinase-1 Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MEK kinase-1 antibody is MEK kinase-1 Antibody (1-9C-2A): sc-449.