MEK-4 Antibody (G-7): sc-376838

MEK-4 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects MEK-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-MEK-4 antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MEK-4, also known as SEK1 or MKK-4, plays a crucial role in cellular signaling by activating both p38 and c-Jun N-terminal kinase (JNK) MAP kinases, which are essential for mediating stress responses, apoptosis, and inflammation. The activation of these kinases helps regulate various cellular processes, including cell differentiation, proliferation, and survival, making MEK-4 an important player in the MAPK signaling pathway. Understanding MEK-4 function and protein interactions can provide insights into diseases such as cancer and neurodegenerative disorders, where dysregulation of these pathways often occurs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MEK-4 Antibody (G-7) References:

1. Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide inhibit the MEKK1/MEK4/JNK signaling pathway in endotoxin-activated microglia.  |  Delgado, M. 2002. Biochem Biophys Res Commun. 293: 771-6. PMID: 12054537
2. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
3. Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases.  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
4. Components of a new human protein kinase signal transduction pathway.  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
5. Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms.  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
6. Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2.  |  Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
7. Characterization of the structure and function of a novel MAP kinase kinase (MKK6).  |  Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
8. Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta).  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
9. Expression of the Tyro4/Mek4/Cek4 gene specifically marks a subset of embryonic motor neurons and their muscle targets.  |  Kilpatrick, TJ., et al. 1996. Mol Cell Neurosci. 7: 62-74. PMID: 8812059
10. Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase.  |  Tournier, C., et al. 1997. Proc Natl Acad Sci U S A. 94: 7337-42. PMID: 9207092
11. MKK7 is a stress-activated mitogen-activated protein kinase kinase functionally related to hemipterous.  |  Holland, PM., et al. 1997. J Biol Chem. 272: 24994-8. PMID: 9312105
12. Molecular cloning and characterization of human JNKK2, a novel Jun NH2-terminal kinase-specific kinase.  |  Wu, Z., et al. 1997. Mol Cell Biol. 17: 7407-16. PMID: 9372971

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEK-4 Antibody (G-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-MEK-4 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEK-4 Antibody (G-7) Specifications

Alternate Product Name: MEK-4

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 43-79

Epitope Target: MEK-4

Epitope Species: mouse

Product Reactivity/Detection: MEK-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for MEK-4 Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin