MEK-3/6 Antibody (B-1): sc-136982

MEK-3/6 Antibody (B-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects MEK-3/6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-MEK-3/6 antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MEK-3/6 (B-1) antibody targets a protein that plays a crucial role in the MAPK signaling pathway, specifically in the activation of p38 MAP kinase and JNK MAP kinases, which are essential for cellular responses to stress and inflammation. MEK-3/6 (B-1) monoclonal antibody recognizes a protein vital for regulating various physiological processes, including cell differentiation, proliferation, and apoptosis. Dysregulation of this pathway can lead to various diseases, including cancer and inflammatory disorders, making MEK-3/6 a significant target for therapeutic intervention. Understanding MEK-3/6 interactions with other proteins in the MAPK pathway can provide insights into regulatory mechanisms and potential roles in disease progression.

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MEK-3/6 Antibody (B-1) References:

1. MEK6 regulates human involucrin gene expression via a p38alpha - and p38delta -dependent mechanism.  |  Dashti, SR., et al. 2001. J Biol Chem. 276: 27214-20. PMID: 11454875
2. Green tea polyphenol stimulates a Ras, MEKK1, MEK3, and p38 cascade to increase activator protein 1 factor-dependent involucrin gene expression in normal human keratinocytes.  |  Balasubramanian, S., et al. 2002. J Biol Chem. 277: 1828-36. PMID: 11698415
3. Tissue inhibitor of metalloproteinase-1 promotes hematopoietic differentiation via caspase-3 upstream the MEKK1/MEK6/p38alpha pathway.  |  Dassé, E., et al. 2007. Leukemia. 21: 595-603. PMID: 17301822
4. Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3.  |  Prickett, TD. and Brautigan, DL. 2007. Mol Cell Biol. 27: 4217-27. PMID: 17438131
5. The protein kinase CK2 inhibitor TBB mediates up-regulation of MEK3/6 and p38δ activities, down-regulation of ERK1/2 activity and induction of G1/S arrest in normal human epidermal autocrine proliferating keratinocytes.  |  Isaeva, AR. and Mitev, VI. 2011. J Dermatol Sci. 63: 124-6. PMID: 21620683
6. Catenarin Prevents Type 1 Diabetes in Nonobese Diabetic Mice via Inhibition of Leukocyte Migration Involving the MEK6/p38 and MEK7/JNK Pathways.  |  Shen, MY., et al. 2012. Evid Based Complement Alternat Med. 2012: 982396. PMID: 22454693
7. MTA2 silencing attenuates the metastatic potential of cervical cancer cells by inhibiting AP1-mediated MMP12 expression via the ASK1/MEK3/p38/YB1 axis.  |  Lin, CL., et al. 2021. Cell Death Dis. 12: 451. PMID: 33958583
8. A novel human specific lncRNA MEK6-AS1 regulates adipogenesis and fatty acid biosynthesis by stabilizing MEK6 mRNA.  |  Li, D., et al. 2025. J Biomed Sci. 32: 6. PMID: 39773638
9. Chromosomal localization of four MAPK signaling cascade genes: MEK1, MEK3, MEK4 and MEKK5.  |  Rampoldi, L., et al. 1997. Cytogenet Cell Genet. 78: 301-3. PMID: 9465908
10. Regulation of human involucrin promoter activity by a protein kinase C, Ras, MEKK1, MEK3, p38/RK, AP1 signal transduction pathway.  |  Efimova, T., et al. 1998. J Biol Chem. 273: 24387-95. PMID: 9733728

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEK-3/6 Antibody (B-1) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-MEK-3/6 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEK-3/6 Antibody (B-1) Specifications

Alternate Product Name: MEK-3/6

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 229-318

Epitope Target: MEK-3/6

Epitope Species: human

Product Reactivity/Detection: MEK-3 and MEK-6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for MEK-3/6 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-MEK-3/6 antibody is p-MEK-3/6 Antibody (B-9): sc-8407.