Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Meis1 CRISPR/Cas9 KO Plasmid (h): sc-401481
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Meis1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Meis1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Meis1/2/3 Antibody (9.2.7): sc-101850
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Meis1 CRISPR/Cas9 KO Plasmid (h) | sc-401481 | 20 µg | $397.00 |
Overview
MEIS1 encodes Meis1, a TALE homeobox transcription factor that cooperates with HOX and PBX proteins to control gene expression programs governing embryonic patterning, hematopoietic differentiation, and cell fate commitment. Meis1 influences chromatin-associated transcriptional networks involved in proliferation, lineage specification, and stem/progenitor maintenance, with context-dependent roles across tissues. Altered MEIS1 regulation has been linked to dysregulated hematopoiesis and oncogenic transcriptional circuitry in leukemia, and genetic association studies implicate MEIS1 in neurobiology-related traits such as restless legs syndrome. As a nuclear regulator, MEIS1 is widely used as a model for studying developmental gene regulatory networks and transcription factor cooperativity.
Meis1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MEIS1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MEIS1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MEIS1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Meis1 protein expression.
This CRISPR knockout system enables efficient generation of MEIS1-deficient cell models for investigation of Meis1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MEIS1 exon(s) critical for Meis1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MEIS1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Meis1 CRISPR/Cas9 KO Plasmid (h) and Meis1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MEIS1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Meis1 HDR Plasmid (h) and Meis1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MEIS1 homology arms to support homology-directed repair at defined MEIS1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.