Meis1/2/3 Antibody (9.2.7): sc-101850

Meis1/2/3 Antibody (9.2.7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Meis1/2/3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Meis1/2/3 (9.2.7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Meis1/2/3 proteins are critical transcription factors that play a significant role in developmental processes and hematopoietic differentiation. Meis1/2/3 proteins are primarily located in the nucleus, where these factors function as essential regulators of gene expression by forming heteromeric complexes with other transcription factors, such as Hox and Pbx proteins. This nuclear localization allows Meis proteins to directly influence transcriptional regulation of target genes involved in cell fate determination and tissue patterning. Notably, interaction between Meis proteins and Hox proteins enhances DNA binding affinity, which is crucial for precise regulation of developmental genes. Aberrant expression of Meis proteins has been implicated in various forms of leukemia, highlighting their importance in both normal physiology and disease states. Formation of distinct complexes between Meis1 and Pbx proteins, such as Pbx1, Pbx2, and Pbx3, further underscores regulatory mechanism complexity and potential as therapeutic targets in oncogenesis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Meis1/2/3 Antibody (9.2.7) References:

1. PBX and MEIS as non-DNA-binding partners in trimeric complexes with HOX proteins.  |  Shanmugam, K., et al. 1999. Mol Cell Biol. 19: 7577-88. PMID: 10523646
2. Frequent co-expression of the HOXA9 and MEIS1 homeobox genes in human myeloid leukemias.  |  Lawrence, HJ., et al. 1999. Leukemia. 13: 1993-9. PMID: 10602420
3. Acceleration of chronic myeloproliferation by enforced expression of Meis1 or Meis3 in Icsbp-deficient bone marrow cells.  |  Hara, T., et al. 2008. Oncogene. 27: 3865-9. PMID: 18223676
4. MicroRNAs regulating meis1 expression and inducing cardiomyocyte proliferation.  |  Pandey, R., et al. 2016. Cardiovasc Regen Med. 3: PMID: 28111633
5. The homeobox transcription factor MEIS2 is a regulator of cancer cell survival and IMiDs activity in Multiple Myeloma: modulation by Bromodomain and Extra-Terminal (BET) protein inhibitors.  |  Abruzzese, MP., et al. 2019. Cell Death Dis. 10: 324. PMID: 30975979
6. Homeobox gene Meis1 modulates cardiovascular regeneration.  |  Paul, S., et al. 2020. Semin Cell Dev Biol. 100: 52-61. PMID: 31623926
7. Downregulation of MEIS1 mediated by ELFN1-AS1/EZH2/DNMT3a axis promotes tumorigenesis and oxaliplatin resistance in colorectal cancer.  |  Li, Y., et al. 2022. Signal Transduct Target Ther. 7: 87. PMID: 35351858
8. Identification of a new family of Pbx-related homeobox genes.  |  Nakamura, T., et al. 1996. Oncogene. 13: 2235-42. PMID: 8950991
9. AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins.  |  Shen, WF., et al. 1997. Mol Cell Biol. 17: 6448-58. PMID: 9343407
10. Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an interaction surface disrupted in oncoprotein E2a-Pbx1.  |  Knoepfler, PS., et al. 1997. Proc Natl Acad Sci U S A. 94: 14553-8. PMID: 9405651
11. Hoxa9 transforms primary bone marrow cells through specific collaboration with Meis1a but not Pbx1b.  |  Kroon, E., et al. 1998. EMBO J. 17: 3714-25. PMID: 9649441
12. An endocrine-exocrine switch in the activity of the pancreatic homeodomain protein PDX1 through formation of a trimeric complex with PBX1b and MRG1 (MEIS2).  |  Swift, GH., et al. 1998. Mol Cell Biol. 18: 5109-20. PMID: 9710595

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Meis1/2/3 Antibody (9.2.7) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Meis1/2/3 Antibody (9.2.7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Meis1/2/3 Antibody (9.2.7) Specifications

Alternate Product Name: MEIS1 / MEIS2 / MEIS3

Product Clone Name: 9.2.7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 59-390

Epitope Target: Meis1

Epitope Species: mouse

Product Reactivity/Detection: Meis1/2/3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Meis1/2/3 Antibody (9.2.7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Meis2 antibody is Meis2 Antibody (63-T): sc-81986.