Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Med8 CRISPR/Cas9 KO Plasmid (h): sc-404402
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Med8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Med8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Med8 Antibody (A-5): sc-365960
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Med8 CRISPR/Cas9 KO Plasmid (h) | sc-404402 | 20 µg | $397.00 |
Overview
MED8 encodes Med8, a conserved subunit of the Mediator complex that bridges sequence-specific transcription factors with RNA polymerase II to coordinate transcription initiation and elongation. Through its role in Mediator integrity and promoter–enhancer communication, MED8 contributes to regulation of cell cycle progression, differentiation programs, and stress-responsive gene expression. Perturbation of Mediator components can rewire transcriptional networks that influence genomic stability and metabolic state, making MED8 relevant for mechanistic studies in cancer biology and other disorders driven by transcriptional dysregulation. Human MED8 is therefore a useful node for interrogating core transcriptional control pathways and context-dependent gene regulatory dependencies.
Med8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MED8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MED8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MED8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Med8 protein expression.
This CRISPR knockout system enables efficient generation of MED8-deficient cell models for investigation of Med8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MED8 exon(s) critical for Med8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MED8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Med8 CRISPR/Cas9 KO Plasmid (h) and Med8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MED8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Med8 HDR Plasmid (h) and Med8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MED8 homology arms to support homology-directed repair at defined MED8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.