Med8 Antibody (A-5): sc-365960
- Med8 Antibody (A-5) is a mouse monoclonal IgG1 κ Med8 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-268 representing full length Med8 of human origin
- Med8 Antibody (A-5) is recommended for detection of Med8 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Med8 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Med8 (A-5): sc-365960.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Med8 Antibody (A-5) for WB applications. This reagent is now offered in a bundle with Med8 Antibody (A-5) (see ordering information below).
Med8 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Med8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Med8 (A-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Med8, also known as mediator complex subunit 8 or arc32, is a crucial 268 amino acid component of the mediator complex, which plays a vital role in regulating transcription in mammalian cells. Med8 is primarily located in the nucleus, where Med8 acts as a bridge between transcriptional activators and RNA polymerase II, facilitating gene transcription. Proper functioning of Med8 is essential for effective gene expression, as Med8 helps coordinate complex interactions necessary for transcriptional regulation. Additionally, Med8 is involved in post-translational modifications and ubiquitination processes, which are critical for degrading proteins that are no longer needed, thus maintaining cellular homeostasis. Notably, multiple isoforms of Med8 arise from alternative splicing, allowing for diverse regulatory functions within the cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Med8 Antibody (A-5) References:
- Composite co-activator ARC mediates chromatin-directed transcriptional activation. | Näär, AM., et al. 1999. Nature. 398: 828-32. PMID: 10235267
- Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. | Brower, CS., et al. 2002. Proc Natl Acad Sci U S A. 99: 10353-8. PMID: 12149480
- Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. | Sato, S., et al. 2003. J Biol Chem. 278: 15123-7. PMID: 12584197
- A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. | Sato, S., et al. 2003. J Biol Chem. 278: 49671-4. PMID: 14576168
- A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. | Tomomori-Sato, C., et al. 2004. J Biol Chem. 279: 5846-51. PMID: 14638676
- A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. | Sato, S., et al. 2004. Mol Cell. 14: 685-91. PMID: 15175163
- Interaction of Bunyamwera Orthobunyavirus NSs protein with mediator protein MED8: a mechanism for inhibiting the interferon response. | Léonard, VH., et al. 2006. J Virol. 80: 9667-75. PMID: 16973571
- Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. | Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Med8 Antibody (A-5) | sc-365960 | 200 µg/ml | $316.00 | |||
Med8 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-525908 | 200 µg Ab; 10 µg BP | $354.00 | |||
Med8 Antibody (A-5) AC | sc-365960 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Med8 Antibody (A-5) HRP | sc-365960 HRP | 200 µg/ml | $316.00 | |||
Med8 Antibody (A-5) FITC | sc-365960 FITC | 200 µg/ml | $330.00 | |||
Med8 Antibody (A-5) PE | sc-365960 PE | 200 µg/ml | $343.00 | |||
Med8 Antibody (A-5) Alexa Fluor® 488 | sc-365960 AF488 | 200 µg/ml | $357.00 | |||
Med8 Antibody (A-5) Alexa Fluor® 546 | sc-365960 AF546 | 200 µg/ml | $357.00 | |||
Med8 Antibody (A-5) Alexa Fluor® 594 | sc-365960 AF594 | 200 µg/ml | $357.00 | |||
Med8 Antibody (A-5) Alexa Fluor® 647 | sc-365960 AF647 | 200 µg/ml | $357.00 | |||
Med8 Antibody (A-5) Alexa Fluor® 680 | sc-365960 AF680 | 200 µg/ml | $357.00 | |||
Med8 Antibody (A-5) Alexa Fluor® 790 | sc-365960 AF790 | 200 µg/ml | $357.00 |