Med6 Antibody (D-2): sc-390474

Med6 Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Med6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Med6 (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Med6 plays a crucial role in regulating transcription within mammalian cells, functioning as a key component of high molecular weight coactivating complexes that facilitate communication between transcriptional activators and RNA polymerase II. Med6 is part of the SMCC (SRB and MED protein cofactor complex), which is essential for enhancing gene-specific activation or repression by DNA-binding transcription factors. SMCC not only associates with RNA polymerase II holoenzyme through Srb7 subunit but also interacts with coactivator proteins from TRAP (thyroid hormone receptor-activating protein) and DRIP (vitamin D receptor interacting protein) complexes, thereby promoting steroid receptor-dependent transcriptional activation. Med6′s interaction with PC4 (positive cofactor 4) highlights a role in repressing basal transcription independently of RNA polymerase II activity, underscoring Med6′s importance in gene expression regulation.

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Med6 Antibody (D-2) References:

1. A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation.  |  Gu, W., et al. 1999. Mol Cell. 3: 97-108. PMID: 10024883
2. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators.  |  Ito, M., et al. 1999. Mol Cell. 3: 361-70. PMID: 10198638
3. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.  |  Rachez, C., et al. 1999. Nature. 398: 824-8. PMID: 10235266
4. Characterization of the influence of mediator complex in HIV-1 transcription.  |  Ruiz, A., et al. 2014. J Biol Chem. 289: 27665-76. PMID: 25100719
5. Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex.  |  Kim, GS. and Lee, YC. 2018. Biochem Res Int. 2018: 6406372. PMID: 29992056
6. ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma.  |  Nasir, A., et al. 2021. Neurooncol Adv. 3: vdab030. PMID: 33948561
7. Identification of diagnostic genes for both Alzheimer's disease and Metabolic syndrome by the machine learning algorithm.  |  Li, J., et al. 2022. Front Immunol. 13: 1037318. PMID: 36405716
8. A new strategy for screening novel functional genes involved in reduction of lipid droplet accumulation.  |  Maruyama, R., et al. 2024. Biofactors. 50: 467-476. PMID: 37983968
9. A dynamic model for PC4 coactivator function in RNA polymerase II transcription.  |  Malik, S., et al. 1998. Proc Natl Acad Sci U S A. 95: 2192-7. PMID: 9482861
10. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways.  |  Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713
11. Requirement for a functional interaction between mediator components Med6 and Srb4 in RNA polymerase II transcription.  |  Lee, YC. and Kim, YJ. 1998. Mol Cell Biol. 18: 5364-70. PMID: 9710620
12. The human homologue of Drosophila TRF-proximal protein is associated with an RNA polymerase II-SRB complex.  |  Xiao, H., et al. 1999. J Biol Chem. 274: 3937-40. PMID: 9933582

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Med6 Antibody (D-2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Med6 Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Med6 Antibody (D-2) Specifications

Alternate Product Name: Med6

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 52-246

Epitope Target: Med6

Epitope Species: human

Product Reactivity/Detection: Med6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Med6 Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent