Med18 Antibody (E-4): sc-514415
- Med18 Antibody (E-4) is a mouse monoclonal IgG1 κ Med18 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-208 representing full length Med18 of human origin
- Med18 Antibody (E-4) is recommended for detection of Med18 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Med18 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Med18 Antibody (E-4) for WB applications. This reagent is now offered in a bundle with Med18 Antibody (E-4) (see ordering information below).
Med18 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Med18 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Med18 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Med18, also known as mediator complex subunit 18 or p28b, is a crucial component of the mediator complex, which plays a vital role in transcription regulation in mammalian cells. This 208 amino acid protein is localized in the nucleus and functions as a scaffold that facilitates assembly of a functional preinitiation complex with RNA polymerase II and general transcription factors, thereby enhancing transcription of RNA polymerase II-dependent genes. Med18 interacts with several other proteins, including Med8 and Med25, to effectively bridge regulatory proteins and RNA polymerase II, ensuring precise transcriptional regulation. Med18′s role in transcriptional activation underscores potential as a target for research into gene expression regulation and implications in various biological processes and diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Med18 Antibody (E-4) References:
- Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. | Rachez, C., et al. 1999. Nature. 398: 824-8. PMID: 10235266
- Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3. | Sato, S., et al. 2003. J Biol Chem. 278: 15123-7. PMID: 12584197
- A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. | Sato, S., et al. 2003. J Biol Chem. 278: 49671-4. PMID: 14576168
- A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. | Tomomori-Sato, C., et al. 2004. J Biol Chem. 279: 5846-51. PMID: 14638676
- A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. | Sato, S., et al. 2004. Mol Cell. 14: 685-91. PMID: 15175163
- The mammalian Mediator complex. | Conaway, JW., et al. 2005. FEBS Lett. 579: 904-8. PMID: 15680972
- Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. | Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Med18 Antibody (E-4) | sc-514415 | 200 µg/ml | $322.00 | |||
Med18 Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-531784 | 200 µg Ab; 20 µg BP | $361.00 | |||
Med18 Antibody (E-4) AC | sc-514415 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Med18 Antibody (E-4) HRP | sc-514415 HRP | 200 µg/ml | $322.00 | |||
Med18 Antibody (E-4) FITC | sc-514415 FITC | 200 µg/ml | $336.00 | |||
Med18 Antibody (E-4) PE | sc-514415 PE | 200 µg/ml | $349.00 | |||
Med18 Antibody (E-4) Alexa Fluor® 488 | sc-514415 AF488 | 200 µg/ml | $364.00 | |||
Med18 Antibody (E-4) Alexa Fluor® 546 | sc-514415 AF546 | 200 µg/ml | $364.00 | |||
Med18 Antibody (E-4) Alexa Fluor® 594 | sc-514415 AF594 | 200 µg/ml | $364.00 | |||
Med18 Antibody (E-4) Alexa Fluor® 647 | sc-514415 AF647 | 200 µg/ml | $364.00 | |||
Med18 Antibody (E-4) Alexa Fluor® 680 | sc-514415 AF680 | 200 µg/ml | $364.00 | |||
Med18 Antibody (E-4) Alexa Fluor® 790 | sc-514415 AF790 | 200 µg/ml | $364.00 |