MECL-1 Antibody (C-2): sc-133236

MECL-1 Antibody (C-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects MECL-1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MECL-1 (C-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MECL-1, also known as multicatalytic endopeptidase complex-like-1, plays a crucial role in forming immunoproteasomes, which are essential for efficient antigen processing and presentation via major histocompatibility complex (MHC) class I molecules. Upon stimulation with interferon-gamma, standard 20S proteasome subunits are replaced by inducible subunits, including MECL-1, leading to the generation of peptide ligands vital for immune response. MECL-1 presence in immunoproteasomes enhances protein cleavage specificity, allowing for the production of peptides more effectively presented to T cells, thereby influencing adaptive immunity. MECL-1′s ability to form distinct proteasome complexes, along with other subunits like LMP2 and LMP7, results in a diverse array of proteasome subsets that can tailor immune response to various pathogens.

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MECL-1 Antibody (C-2) References:

1. Efficient generation of a hepatitis B virus cytotoxic T lymphocyte epitope requires the structural features of immunoproteasomes.  |  Sijts, AJ., et al. 2000. J Exp Med. 191: 503-14. PMID: 10662796
2. Overexpression of the proteasome subunits LMP2, LMP7, and MECL-1, but not PA28 alpha/beta, enhances the presentation of an immunodominant lymphocytic choriomeningitis virus T cell epitope.  |  Schwarz, K., et al. 2000. J Immunol. 165: 768-78. PMID: 10878350
3. Discrete cleavage motifs of constitutive and immunoproteasomes revealed by quantitative analysis of cleavage products.  |  Toes, RE., et al. 2001. J Exp Med. 194: 1-12. PMID: 11435468
4. T cells lacking immunoproteasome subunits MECL-1 and LMP7 hyperproliferate in response to polyclonal mitogens.  |  Caudill, CM., et al. 2006. J Immunol. 176: 4075-82. PMID: 16547243
5. Autoimmune reactivity against the 20S-proteasome includes immunosubunits LMP2 (beta1i), MECL1 (beta2i) and LMP7 (beta5i).  |  Scheffler, S., et al. 2008. Rheumatology (Oxford). 47: 622-6. PMID: 18375405
6. Adenovirus E1A interacts directly with, and regulates the level of expression of, the immunoproteasome component MECL1.  |  Berhane, S., et al. 2011. Virology. 421: 149-58. PMID: 22018786
7. The Immunoproteasome Subunits LMP2, LMP7 and MECL-1 Are Crucial Along the Induction of Cerebral Toxoplasmosis.  |  French, T., et al. 2021. Front Immunol. 12: 619465. PMID: 33968021
8. [Not Available].  |  Inholz, K., et al. 2024. Eur J Immunol. 54: e2350613. PMID: 38458995
9. Identification of MECL-1 (LMP-10) as the third IFN-gamma-inducible proteasome subunit.  |  Nandi, D., et al. 1996. J Immunol. 156: 2361-4. PMID: 8786291
10. Molecular cloning of the mouse proteasome subunits MC14 and MECL-1: reciprocally regulated tissue expression of interferon-gamma-modulated proteasome subunits.  |  Stohwasser, R., et al. 1997. Eur J Immunol. 27: 1182-7. PMID: 9174609
11. The subunits MECL-1 and LMP2 are mutually required for incorporation into the 20S proteasome.  |  Groettrup, M., et al. 1997. Proc Natl Acad Sci U S A. 94: 8970-5. PMID: 9256419
12. Phenotype-dependent differences in proteasome subunit composition and cleavage specificity in B cell lines.  |  Frisan, T., et al. 1998. J Immunol. 160: 3281-9. PMID: 9531285

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MECL-1 Antibody (C-2) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-MECL-1 Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MECL-1 Antibody (C-2) Specifications

Alternate Product Name: MECL-1

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 139-208

Epitope Target: MECL-1

Epitope Species: human

Product Reactivity/Detection: MECL-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for MECL-1 Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin