ME1 Antibody (99.1): sc-100569

ME1 Antibody (99.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ME1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-ME1 antibody (99.1) is available in a non-conjugated form, providing flexibility for various experimental needs. ME1, or Malic enzyme 1, is a 572 amino acid cytoplasmic protein that plays a crucial role in cellular metabolism as an NADP-dependent enzyme, catalyzing the conversion of S-malate to pyruvate, carbon dioxide, and NADPH, which is essential for fatty acid biosynthesis. ME1 is predominantly expressed in the liver and white adipose tissue, linking the citric acid and glycolytic cycles through its ability to facilitate the reversible oxidative decarboxylation of malate. Notably, ME1 exists as a homotetramer and requires divalent metal cations, such as magnesium or manganese, as cofactors for enzymatic activity. The regulation of ME1 expression by thyroid hormone levels and dietary carbohydrates highlights its significance as a housekeeping protein, maintaining metabolic homeostasis within the cell.

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ME1 Antibody (99.1) References:

1. Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate.  |  Yang, Z., et al. 2002. Structure. 10: 951-60. PMID: 12121650
2. Determinants of the dual cofactor specificity and substrate cooperativity of the human mitochondrial NAD(P)+-dependent malic enzyme: functional roles of glutamine 362.  |  Hsieh, JY., et al. 2006. J Biol Chem. 281: 23237-45. PMID: 16757477
3. Human malic enzymes in heart and muscle: evidence of a selective distribution.  |  Tessarolo, D., et al. 1991. Biochem Med Metab Biol. 45: 1-5. PMID: 2015102
4. ME1 Regulates NADPH Homeostasis to Promote Gastric Cancer Growth and Metastasis.  |  Lu, YX., et al. 2018. Cancer Res. 78: 1972-1985. PMID: 29654155
5. Dynamic Regulation of ME1 Phosphorylation and Acetylation Affects Lipid Metabolism and Colorectal Tumorigenesis.  |  Zhu, Y., et al. 2020. Mol Cell. 77: 138-149.e5. PMID: 31735643
6. Malic enzyme 1 (ME1) in the biology of cancer: it is not just intermediary metabolism.  |  Simmen, FA., et al. 2020. J Mol Endocrinol. 65: R77-R90. PMID: 33064660
7. USP19 exacerbates lipogenesis and colorectal carcinogenesis by stabilizing ME1.  |  Zhu, Y., et al. 2021. Cell Rep. 37: 110174. PMID: 34965422
8. Salvage therapy expands highly cytotoxic and metabolically fit resilient CD8+ T cells via ME1 up-regulation.  |  Gicobi, JK., et al. 2023. Sci Adv. 9: eadi2414. PMID: 37967193
9. Mediating Metabolism: Inhibition of Malic Enzyme 1 (ME1) Restores Endothelial Bioenergetics and Adenosine Signaling in Pulmonary Hypertension.  |  Rao, RJ. and Chan, SY. 2024. Circulation. 149: 1372-1374. PMID: 38648276
10. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver.  |  González-Manchón, C., et al. 1995. Gene. 159: 255-60. PMID: 7622060
11. Characterization of cytosolic malic enzyme in human tumor cells.  |  Loeber, G., et al. 1994. FEBS Lett. 344: 181-6. PMID: 8187880
12. Molecular cloning and functional characterization of the human cytosolic malic enzyme promoter: thyroid hormone responsiveness.  |  González-Manchón, C., et al. 1997. DNA Cell Biol. 16: 533-44. PMID: 9174159

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ME1 Antibody (99.1) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-ME1 Antibody (99.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ME1 Antibody (99.1) Specifications

Alternate Product Name: ME1

Product Clone Name: 99.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-573

Epitope Target: ME1

Epitope Species: human

Product Reactivity/Detection: ME1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for ME1 Antibody (99.1): N/A

The preferred ME1 antibody is ME1 Antibody (C-6): sc-365891.