MDMX Antibody (G-10): sc-74467

MDMX Antibody (G-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects MDMX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MDMX antibody (G-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MDMX protein plays a crucial role in regulating p53 protein stability, which is essential for maintaining cellular integrity and preventing tumorigenesis. MDMX, an MDM2-related protein, inhibits p53 degradation mediated by MDM2 through distinct interactions, thereby influencing p53′s function in cell cycle regulation and apoptosis. MDMX (also known as MDM4) is frequently amplified in malignant gliomas, highlighting its significance in cancer biology. Additionally, MDMX interacts with ARF protein, which sequesters MDMX in the nucleolus, leading to enhanced p53 transactivation and increased p53 activity. This dynamic interplay between MDMX, MDM2, and p53 underscores MDMX′s importance in regulating critical cellular processes and potential as a therapeutic target in cancer treatment.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MDMX Antibody (G-10) References:

1. Targeting p53-MDM2-MDMX loop for cancer therapy.  |  Zhang, Q., et al. 2014. Subcell Biochem. 85: 281-319. PMID: 25201201
2. The Roles of MDM2 and MDMX in Cancer.  |  Karni-Schmidt, O., et al. 2016. Annu Rev Pathol. 11: 617-44. PMID: 27022975
3. Mouse modelling of the MDM2/MDMX-p53 signalling axis.  |  Tackmann, NR. and Zhang, Y. 2017. J Mol Cell Biol. 9: 34-44. PMID: 28096294
4. MDM2 and MDMX promote ferroptosis by PPARα-mediated lipid remodeling.  |  Venkatesh, D., et al. 2020. Genes Dev. 34: 526-543. PMID: 32079652
5. MDMX acts as a pervasive preleukemic-to-acute myeloid leukemia transition mechanism.  |  Ueda, K., et al. 2021. Cancer Cell. 39: 529-547.e7. PMID: 33667384
6. MDMX drives pre-leukaemia progression.  |  Seton-Rogers, S. 2021. Nat Rev Cancer. 21: 280. PMID: 33762752
7. The role of miRNAs in MDMX-p53 interplay.  |  Yu, D., et al. 2021. J Evid Based Med. 14: 152-160. PMID: 33988919
8. Therapeutic opportunities in cancer therapy: targeting the p53-MDM2/MDMX interactions.  |  Munisamy, M., et al. 2021. Am J Cancer Res. 11: 5762-5781. PMID: 35018225
9. The MDM2/MDMX/p53 axis in the adaptive stress response.  |  Wang, B., et al. 2020. Transl Cancer Res. 9: 1993-1997. PMID: 35117546
10. Verbenalin attenuates hepatic damage and mitochondrial dysfunction in alcohol-associated steatohepatitis by regulating MDMX/PPARα-mediated ferroptosis.  |  Dong, J., et al. 2023. J Ethnopharmacol. 307: 116227. PMID: 36739928

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MDMX Antibody (G-10) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-MDMX Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MDMX Antibody (G-10) Specifications

Alternate Product Name: MDM4

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 361-490

Epitope Target: MDMX

Epitope Species: human

Product Reactivity/Detection: MDMX

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for MDMX Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MDMX antibody is MDMX Antibody (D-4): sc-74468.