MDMX Antibody (D-4): sc-74468

MDMX Antibody (D-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects MDMX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MDMX antibody (D-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MDMX protein plays a crucial role in regulating p53 tumor suppressor pathway, which is vital for maintaining genomic stability and preventing tumorigenesis. MDMX functions by inhibiting p53 degradation mediated by MDM2, thereby stabilizing p53 levels and allowing p53 to perform essential roles in cell cycle regulation and apoptosis. Notably, the gene encoding MDMX, also known as MDM4, is frequently amplified in malignant gliomas, highlighting MDMX significance in cancer biology. MDMX interacts with ARF protein, which sequesters MDMX in the nucleolus, leading to enhanced p53 transactivation and subsequent increase in p53 activity. This intricate interplay between MDMX, MDM2, and p53 underscores MDMX importance in cellular responses to stress and potential as a therapeutic target in cancer treatment.

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MDMX Antibody (D-4) References:

1. MDM2 and MDMX bind and stabilize the p53-related protein p73.  |  Ongkeko, WM., et al. Curr Biol. 9: 829-32. PMID: 10469568
2. Amplification and overexpression of the MDM4 (MDMX) gene from 1q32 in a subset of malignant gliomas without TP53 mutation or MDM2 amplification.  |  Riemenschneider, MJ., et al. 1999. Cancer Res. 59: 6091-6. PMID: 10626796
3. MdmX protects p53 from Mdm2-mediated degradation.  |  Jackson, MW. and Berberich, SJ. 2000. Mol Cell Biol. 20: 1001-7. PMID: 10629057
4. MDM2 and MDMX can interact differently with ARF and members of the p53 family.  |  Wang, X., et al. 2001. FEBS Lett. 490: 202-8. PMID: 11223036
5. MdmX binding to ARF affects Mdm2 protein stability and p53 transactivation.  |  Jackson, MW., et al. 2001. J Biol Chem. 276: 25336-41. PMID: 11297540
6. An essential function of the extreme C-terminus of MDM2 can be provided by MDMX.  |  Uldrijan, S., et al. 2007. EMBO J. 26: 102-12. PMID: 17159902
7. MDMX: from bench to bedside.  |  Marine, JC., et al. 2007. J Cell Sci. 120: 371-8. PMID: 17251377
8. Hetero-oligomerization with MdmX rescues the ubiquitin/Nedd8 ligase activity of RING finger mutants of Mdm2.  |  Singh, RK., et al. 2007. J Biol Chem. 282: 10901-7. PMID: 17301054
9. Distinct roles of MDMX in the regulation of p53 response to ribosomal stress.  |  Gilkes, DM. and Chen, J. 2007. Cell Cycle. 6: 151-5. PMID: 17327702
10. MdmX regulates transformation and chromosomal stability in p53-deficient cells.  |  Matijasevic, Z., et al. 2008. Cell Cycle. 7: 2967-73. PMID: 18818521
11. Medicinal Chemistry Strategies to Disrupt the p53-MDM2/MDMX Interaction.  |  Lemos, A., et al. 2016. Med Res Rev. 36: 789-844. PMID: 27302609
12. Targeting MDMX for Cancer Therapy: Rationale, Strategies, and Challenges.  |  Yu, DH., et al. 2020. Front Oncol. 10: 1389. PMID: 32850448

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MDMX Antibody (D-4) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-MDMX Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MDMX Antibody (D-4) Specifications

Alternate Product Name: MDMX

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 361-490

Epitope Target: MDMX

Epitope Species: human

Product Reactivity/Detection: MDMX

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for MDMX Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin