MDHC Antibody (H-6): sc-166879

MDHC Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects MDHC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MDHC (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The cytosolic malate dehydrogenase (MDHC) protein plays a crucial role in cellular metabolism as an NAD-dependent enzyme that catalyzes the conversion of L-malate and NAD into oxaloacetate and NADH, which is vital for energy production. MDHC protein is predominantly located in the cytosol of cells, particularly in tissues such as the brain, heart, and skeletal muscle, where MDHC supports aerobic energy production necessary for muscle contraction and neuronal signaling. MDHC protein is involved in various metabolic pathways, including those related to collagen synthesis, cell division, and gas exchange, highlighting MDHC′s importance in maintaining cellular homeostasis and function. Hormones such as juvenile hormone and ecdysone regulate MDHC activity, which further underscores MDHC′s significance in metabolic regulation. With high conservation across species, MDHC protein serves as a key player in fundamental biological processes, making anti-MDHC antibody (H-6) an essential tool for researchers studying metabolic pathways and energy production in various organisms.

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MDHC Antibody (H-6) References:

1. Ability of cytosolic malate dehydrogenase and lactate dehydrogenase to increase the ratio of NADPH to NADH oxidation by cytosolic glycerol-3-phosphate dehydrogenase.  |  Fahien, LA., et al. 1999. Arch Biochem Biophys. 364: 185-94. PMID: 10190973
2. Cytosolic malate dehydrogenase confers selectivity of the nucleic acid-conducting channel.  |  Hanss, B., et al. 2002. Proc Natl Acad Sci U S A. 99: 1707-12. PMID: 11805283
3. Evolution of the vertebrate cytosolic malate dehydrogenase gene family: duplication and divergence in actinopterygian fish.  |  Merrit, TJ. and Quattro, M. 2003. J Mol Evol. 56: 265-76. PMID: 12612830
4. dl-alpha-lipoic acid ameliorates cyclophosphamide induced cardiac mitochondrial injury.  |  Mythili, Y., et al. 2005. Toxicology. 215: 108-14. PMID: 16085348
5. Genetic variability among beech (Fagus sylvatica L.) populations from the Sudety Mountains, in respect of peroxidase and malate dehydrogenase loci.  |  Krzakowa, M. and Matras, J. 2005. J Appl Genet. 46: 271-7. PMID: 16110183
6. Modulation of TCA cycle enzymes and electron transport chain systems in experimental lung cancer.  |  Senthilnathan, P., et al. 2006. Life Sci. 78: 1010-4. PMID: 16143346
7. Neutron scattering reveals the dynamic basis of protein adaptation to extreme temperature.  |  Tehei, M., et al. 2005. J Biol Chem. 280: 40974-9. PMID: 16203729
8. Quantitative expression proteomics and phosphoproteomics profile of brain from PINK1 knockout mice: insights into mechanisms of familial Parkinson's disease.  |  Triplett, JC., et al. 2015. J Neurochem. 133: 750-65. PMID: 25626353
9. Machine-learning algorithms for asthma, COPD, and lung cancer risk assessment using circulating microbial extracellular vesicle data and their application to assess dietary effects.  |  McDowell, A., et al. 2022. Exp Mol Med. 54: 1586-1595. PMID: 36180580
10. Suppression of tyrosine kinase activity inhibits [3H]thymidine uptake in cultured human pituitary tumor cells.  |  Jones, TH., et al. 1997. J Clin Endocrinol Metab. 82: 2143-7. PMID: 9215285
11. Isolation and characterization of cytosolic malate dehydrogenase from Trichomonas vaginalis.  |  Drmota, T., et al. 1997. Folia Parasitol (Praha). 44: 103-8. PMID: 9269720
12. Genetic and hormonal control of cytosolic malate dehydrogenase activity in Drosophila melanogaster.  |  Farkas, R. and Knopp, J. 1998. Gen Physiol Biophys. 17: 37-50. PMID: 9675555

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MDHC Antibody (H-6) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-MDHC Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MDHC Antibody (H-6) Specifications

Alternate Product Name: MDHC

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 255-320

Epitope Target: MDHC

Epitope Species: human

Product Reactivity/Detection: MDHC (malate dehydrogenase, cytoplasmic)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for MDHC Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin