MDH2 Antibody (1G12): sc-293474

MDH2 Antibody (1G12) is a mouse monoclonal IgG2a kappa light chain antibody that detects MDH2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-MDH2 antibody (1G12) is available as a non-conjugated format. MDH2, also known as malate dehydrogenase, NAD mitochondrial, or M-MDH, consists of 338 amino acids and is a member of the LDH/MDH superfamily. MDH2 is primarily localized in the mitochondria, where MDH2 plays a vital role in the malate-aspartate shuttle, a key mechanism for metabolic coordination between the cytosol and mitochondria. This shuttle is essential for maintaining cellular energy homeostasis, as MDH2 facilitates the transfer of reducing equivalents and metabolites, thereby supporting the citric acid cycle. MDH2 utilizes the NAD/NADH cofactor system to catalyze the reversible oxidation of malate to oxaloacetate, a crucial intermediate involved in various metabolic pathways, including amino acid synthesis and gluconeogenesis. Proper functioning of MDH2 is critical for efficient energy production and metabolic regulation, making MDH2 an important target for research in cellular metabolism and related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MDH2 Antibody (1G12) References:

1. Cat8 and Sip4 mediate regulated transcriptional activation of the yeast malate dehydrogenase gene MDH2 by three carbon source-responsive promoter elements.  |  Roth, S. and Schüller, HJ. 2001. Yeast. 18: 151-62. PMID: 11169757
2. [Subcellular localization of isozymes of NAD-dependent malate dehydrogenase in sugar beet Beta vulgaris L].  |  Iudina, RS. and Levites, EV. 2008. Genetika. 44: 1638-43. PMID: 19178082
3. Palmitoylation of MDH2 by ZDHHC18 activates mitochondrial respiration and accelerates ovarian cancer growth.  |  Pei, X., et al. 2022. Sci China Life Sci. 65: 2017-2030. PMID: 35366151
4. Dexmedetomidine Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting MDH2 Lactylation via Regulating Metabolic Reprogramming.  |  She, H., et al. 2024. Adv Sci (Weinh). 11: e2409499. PMID: 39467114
5. Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification.  |  Mao, Z., et al. 2025. Signal Transduct Target Ther. 10: 67. PMID: 39962087
6. [Malate dehydrogenase and its isoenzymes in the peripheral blood leukocytes in progressive muscular dystrophy of the Duchenne type].  |  Wiśniewska, W. and Lukasiuk, M. 1985. Neurol Neurochir Pol. 19: 318-22. PMID: 4094644
7. Tissue origin of MDH isozymes in blood serum of rats exposed to alkylmercurials.  |  Breźnicka, EA., et al. 1983. J Appl Toxicol. 3: 180-4. PMID: 6643915
8. Expression and function of a mislocalized form of peroxisomal malate dehydrogenase (MDH3) in yeast.  |  McAlister-Henn, L., et al. 1995. J Biol Chem. 270: 21220-5. PMID: 7673155
9. Malate dehydrogenase: a model for structure, evolution, and catalysis.  |  Goward, CR. and Nicholls, DJ. 1994. Protein Sci. 3: 1883-8. PMID: 7849603
10. Glucose-induced phosphorylation of the MDH2 isozyme of malate dehydrogenase in Saccharomyces cerevisiae.  |  Minard, KI. and McAlister-Henn, L. 1994. Arch Biochem Biophys. 315: 302-9. PMID: 7986072
11. A novel automated assay for malate dehydrogenase isoenzymes.  |  Sugiuchi, H., et al. 1996. J Clin Lab Anal. 10: 78-84. PMID: 8852359
12. Overexpression of cytosolic malate dehydrogenase (MDH2) causes overproduction of specific organic acids in Saccharomyces cerevisiae.  |  Pines, O., et al. 1997. Appl Microbiol Biotechnol. 48: 248-55. PMID: 9299784

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MDH2 Antibody (1G12) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-MDH2 Antibody (1G12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MDH2 Antibody (1G12) Specifications

Alternate Product Name: MDH2

Product Clone Name: 1G12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 134-246

Epitope Target: MDH2

Epitope Species: human

Product Reactivity/Detection: MDH2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for MDH2 Antibody (1G12): N/A