MCT4 Antibody (D-1): sc-376140

MCT4 Antibody (D-1) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect Monocarboxylate Transporter 4 (MCT4) in mouse, rat, and human species through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-MCT4 antibody (D-1) specifically targets amino acids 376-465 within the C-terminal cytoplasmic domain of human MCT4, ensuring precise binding and reliable detection. MCT4 is a member of the solute carrier 16A (SLC16A) family of proteins, and is an integral membrane protein characterized by 10-12 transmembrane-helical domains, with both amino and carboxy termini located in the cytoplasm. This structural configuration facilitates proton-linked transport of lactate and other monocarboxylates across the plasma membrane. MCT4 (D-1) antibody plays a vital role in studying intracellular pH balance and sustained glycolytic activity, particularly in highly glycolytic tissues such as skeletal muscle and cancer cells where rapid lactate production is driven by the Warburg effect. Strategic localization of MCT4 on the plasma membrane effectively manages lactate levels, preventing intracellular acidification and supporting cellular metabolism and survival under anaerobic conditions. Understanding MCT4 structure underpins research into cancer metabolism and other diseases characterized by altered lactate transport. Anti-MCT4 antibody (D-1) is available in non-conjugated form and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatile options for diverse experimental needs. MCT4 monoclonal antibody (D-1) supports research on expression, localization, and functional roles of MCT4 in various physiological and pathological contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MCT4 Antibody (D-1) References:

1. Lactate transport in skeletal muscle - role and regulation of the monocarboxylate transporter.  |  Juel, C. and Halestrap, AP. 1999. J Physiol. 517 (Pt 3): 633-42. PMID: 10358105
2. The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation.  |  Halestrap, AP. and Price, NT. 1999. Biochem J. 343 Pt 2: 281-99. PMID: 10510291
3. Lactate transporters (MCT proteins) in heart and skeletal muscles.  |  Bonen, A. 2000. Med Sci Sports Exerc. 32: 778-89. PMID: 10776897
4. Abundance and subcellular distribution of MCT1 and MCT4 in heart and fast-twitch skeletal muscles.  |  Bonen, A., et al. 2000. Am J Physiol Endocrinol Metab. 278: E1067-77. PMID: 10827010
5. Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats.  |  Gerhart, DZ., et al. 1997. Am J Physiol. 273: E207-13. PMID: 9252498
6. Lactate transport in heart in relation to myocardial ischemia.  |  Halestrap, AP., et al. 1997. Am J Cardiol. 80: 17A-25A. PMID: 9293952
7. Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past.  |  Price, NT., et al. 1998. Biochem J. 329 (Pt 2): 321-8. PMID: 9425115
8. Human monocarboxylate transporter 2 (MCT2) is a high affinity pyruvate transporter.  |  Lin, RY., et al. 1998. J Biol Chem. 273: 28959-65. PMID: 9786900

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MCT4 Antibody (D-1) has 83 citations in a variety of scientific publications.

Primary antibodies like Anti-MCT4 Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MCT4 Antibody (D-1) Specifications

Alternate Product Name: SLC16A3

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 376-465

Epitope Target: MCT4

Epitope Species: human

Product Reactivity/Detection: MCT4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 83 citations in various scientific publications

Available Conjugates/Forms for MCT4 Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin