MCP-4 Antibody (D-2): sc-271124

MCP-4 Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects MCP-4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MCP-4 (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MCP-4, also known as CCL13, is a member of the C-C chemokine subfamily, which is characterized by a unique arrangement of conserved cysteine residues. MCP-4 plays a crucial role in the immune response by acting as a potent chemoattractant for monocytes, eosinophils, and basophils, particularly during allergic and non-allergic inflammatory processes. MCP-4 signals through CCR-2 and CCR-3 receptors, facilitating immune cell recruitment to sites of inflammation. MCP-4′s selective attraction of different lymphocyte subpopulations highlights its importance in modulating immune responses and potential implications in various inflammatory diseases. Production of MCP-4 is induced in T lymphocytes following activation by cytokines such as interleukin-1, tumor necrosis factor-alpha, and interferon-gamma, underscoring MCP-4′s role in the dynamic interplay between immune cells and inflammatory mediators.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MCP-4 Antibody (D-2) References:

1. Monocyte chemoattractant protein-4 (MCP-4)/CCL13 is highly expressed in cartilage from patients with rheumatoid arthritis.  |  Iwamoto, T., et al. 2006. Rheumatology (Oxford). 45: 421-4. PMID: 16303818
2. Prognostic biomarker MCP-4 triggers epithelial-mesenchymal transition via the p38 MAPK pathway in ovarian cancer.  |  Li, S., et al. 2022. Front Oncol. 12: 1034737. PMID: 36531002
3. MCP-4 and Eotaxin-3 Are Novel Biomarkers for Chronic Obstructive Pulmonary Disease.  |  Fang, C., et al. 2023. Can Respir J. 2023: 8659293. PMID: 37200921
4. Altered serum TNF-α and MCP-4 levels are associated with the pathophysiology of major depressive disorder: A case-control study results.  |  Nayem, J., et al. 2023. PLoS One. 18: e0294288. PMID: 37967104
5. Monocyte chemotactic protein MCP-2 activates human basophil and eosinophil leukocytes similar to MCP-3.  |  Weber, M., et al. 1995. J Immunol. 154: 4166-72. PMID: 7535823
6. Monocyte chemoattractant protein-3 is a functional ligand for CC chemokine receptors 1 and 2B.  |  Combadiere, C., et al. 1995. J Biol Chem. 270: 29671-5. PMID: 8530354
7. Human monocyte chemotactic proteins-2 and -3: structural and functional comparison with MCP-1.  |  Proost, P., et al. 1996. J Leukoc Biol. 59: 67-74. PMID: 8558070
8. Chemokines in seasonal allergic rhinitis.  |  Kuna, P., et al. 1996. J Allergy Clin Immunol. 97: 104-12. PMID: 8568122
9. Early signal transduction by the receptor to the chemokine monocyte chemotactic protein-1 in a murine T cell hybrid.  |  Dubois, PM., et al. 1996. J Immunol. 156: 1356-61. PMID: 8568234
10. Site-directed mutagenesis of monocyte chemoattractant protein-1 identifies two regions of the polypeptide essential for biological activity.  |  Beall, CJ., et al. 1996. Biochem J. 313 (Pt 2): 633-40. PMID: 8573103
11. Distinct expression and function of the novel mouse chemokine monocyte chemotactic protein-5 in lung allergic inflammation.  |  Jia, GQ., et al. 1996. J Exp Med. 184: 1939-51. PMID: 8920881
12. Human monocyte chemoattractant protein (MCP)-4 is a novel CC chemokine with activities on monocytes, eosinophils, and basophils induced in allergic and nonallergic inflammation that signals through the CC chemokine receptors (CCR)-2 and -3.  |  Garcia-Zepeda, EA., et al. 1996. J Immunol. 157: 5613-26. PMID: 8955214

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MCP-4 Antibody (D-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MCP-4 Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MCP-4 Antibody (D-2) Specifications

Alternate Product Name: CCL13 (MCP-4)

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 69-102

Epitope Target: MCP-4

Epitope Species: human

Product Reactivity/Detection: MCP-4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MCP-4 Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred MCP-1-4/eotaxin antibody is MCP-1-4/eotaxin Antibody (B-2): sc-377082.