Mcm4 Antibody (C-12): sc-166036
- Mcm4 Antibody (C-12) is a mouse monoclonal IgG2a κ Mcm4 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of Mcm4 of Saccharomyces cerevisiae origin
- Mcm4 Antibody (C-12) is recommended for detection of Mcm4 of y origin by WB, IP, IF and ELISA
- Anti-Mcm4 Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Mcm4 Antibody (C-12) for WB applications. These reagents are now offered in bundles with Mcm4 Antibody (C-12) (see ordering information below).
Mcm4 Antibody (C-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Mcm4 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Mcm4 antibody (C-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The mini-chromosome maintenance (MCM) family of proteins, which includes MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5), and MCM7 (Cdc47), plays a crucial role in regulating DNA replication, ensuring that replication occurs only once during each cell cycle. MCM4 is essential for the formation of the MCM complex, which acts as a helicase during DNA unwinding, a critical step for DNA replication. Proper functioning of MCM4 is vital, as any dysregulation can lead to genomic instability and contribute to various diseases, including cancer. Expression of MCM proteins, including MCM4, increases during cell growth, peaking at the transition from G1 to S phase of the cell cycle, and is tightly regulated by E2F transcription factors and protein kinases. MCM4′s interaction with other MCM proteins, particularly in the formation of complexes that facilitate DNA helicase activity, underscores its importance in maintaining genome integrity during cell division.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Mcm4 Antibody (C-12) References:
- miR-31 promotes proliferation of colon cancer cells by targeting E2F2. | Li, T., et al. 2015. Biotechnol Lett. 37: 523-32. PMID: 25362258
- A genome-wide comprehensive analysis of alterations in driver genes in non-small-cell lung cancer. | Yi, J., et al. 2018. Anticancer Drugs. 29: 10-18. PMID: 29084003
- Regulation of MCM2-7 function. | Ishimi, Y. 2018. Genes Genet Syst. 93: 125-133. PMID: 30369561
- Does Natural Killer Cell Deficiency (NKD) Increase the Risk of Cancer? NKD May Increase the Risk of Some Virus Induced Cancer. | Moon, WY. and Powis, SJ. 2019. Front Immunol. 10: 1703. PMID: 31379882
- Antitumor Activity of Ohmyungsamycin A through the Regulation of the Skp2-p27 Axis and MCM4 in Human Colorectal Cancer Cells. | Byun, WS., et al. 2020. J Nat Prod. 83: 118-126. PMID: 31894983
- Comprehensive Analysis of the Expression and Prognosis for MCM4 in Uterine Corpus Endometrial Carcinoma. | Pei, LP., et al. 2022. Front Genet. 13: 890591. PMID: 35719366
- Expression of MCMs in Endometrial Cancer and its Biological Correlation Analysis. | Zhen, Y., et al. 2023. Comb Chem High Throughput Screen. 26: 815-825. PMID: 35792121
- Identification of EGFR-Related LINC00460/mir-338-3p/MCM4 Regulatory Axis as Diagnostic and Prognostic Biomarker of Lung Adenocarcinoma Based on Comprehensive Bioinformatics Analysis and Experimental Validation. | Jia, M., et al. 2022. Cancers (Basel). 14: PMID: 36291859
- Deciphering cervical cancer-associated biomarkers by integrated multi-omics approach. | Li, X., et al. 2022. Am J Transl Res. 14: 8843-8861. PMID: 36628250
- Identification of MCM4 and PRKDC as new regulators of osteosarcoma cell dormancy based on 3D cell cultures. | Jubelin, C., et al. 2024. Biochim Biophys Acta Mol Cell Res. 1871: 119660. PMID: 38216092
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mcm4 Antibody (C-12) | sc-166036 | 200 µg/ml | $322.00 | |||
Mcm4 Antibody (C-12): m-IgG Fc BP-HRP Bundle | sc-528897 | 200 µg Ab; 10 µg BP | $361.00 | |||
Mcm4 Antibody (C-12): m-IgGκ BP-HRP Bundle | sc-521492 | 200 µg Ab, 40 µg BP | $361.00 | |||
Mcm4 Antibody (C-12): m-IgG2a BP-HRP Bundle | sc-547200 | 200 µg Ab; 10 µg BP | $361.00 | |||
Mcm4 Antibody (C-12) AC | sc-166036 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Mcm4 Antibody (C-12) HRP | sc-166036 HRP | 200 µg/ml | $322.00 | |||
Mcm4 Antibody (C-12) FITC | sc-166036 FITC | 200 µg/ml | $336.00 | |||
Mcm4 Antibody (C-12) PE | sc-166036 PE | 200 µg/ml | $349.00 | |||
Mcm4 Antibody (C-12) Alexa Fluor® 488 | sc-166036 AF488 | 200 µg/ml | $364.00 | |||
Mcm4 Antibody (C-12) Alexa Fluor® 546 | sc-166036 AF546 | 200 µg/ml | $364.00 | |||
Mcm4 Antibody (C-12) Alexa Fluor® 594 | sc-166036 AF594 | 200 µg/ml | $364.00 | |||
Mcm4 Antibody (C-12) Alexa Fluor® 647 | sc-166036 AF647 | 200 µg/ml | $364.00 | |||
Mcm4 Antibody (C-12) Alexa Fluor® 680 | sc-166036 AF680 | 200 µg/ml | $364.00 | |||
Mcm4 Antibody (C-12) Alexa Fluor® 790 | sc-166036 AF790 | 200 µg/ml | $364.00 |