Mcl-1 Antibody (MCL701): sc-69838

Mcl-1 Antibody (MCL701) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to detect Mcl-1 proteins from human origin. anti-Mcl-1 antibody (MCL701) targets the full-length recombinant Mcl-1, enabling reliable detection in human samples through various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Mcl-1, also called Myeloid cell leukemia-1, Mcl1, and Gm52627, is a pivotal anti-apoptotic member of the Bcl-2 family that resides primarily in the mitochondria, where Mcl-1 plays a crucial role in regulating cell survival and apoptosis. By inhibiting pro-apoptotic proteins like Bak and Bax, Mcl-1 prevents mitochondrial outer membrane permeabilization and the subsequent release of cytochrome c, thereby safeguarding cells from programmed cell death. The mitochondrial localization of Mcl-1 is essential for function, as this allows immediate interaction with key apoptotic regulators at the site where apoptotic signals are initiated. Additionally, Mcl-1 undergoes tight regulation through various post-translational modifications, including phosphorylation by Glycogen Synthase Kinase 3 (GSK3), which targets Mcl-1 for ubiquitination and proteasomal degradation, a process modulated by survival signals from the PI3K/Akt pathway. Mcl-1 also interacts with BH3-only proteins such as Bim, Noxa, and Puma, which can neutralize anti-apoptotic activity, and with other Bcl-2 family members like Bcl-xL and Bcl-w, maintaining a delicate balance between cell survival and apoptosis. In cancer research, overexpression of Mcl-1 is often associated with resistance to chemotherapy and poor prognosis in various malignancies, including leukemia, lymphoma, and solid tumors. Furthermore, studies in mice have demonstrated that Mcl-1 is essential for the survival of early hematopoietic progenitors, as Mcl-1 deficiency leads to early embryonic lethality and significant reductions in B and T lymphocytes, highlighting an indispensable role in cellular homeostasis and immune function.

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Mcl-1 Antibody (MCL701) References:

1. Small-Molecule and Peptide Inhibitors of the Pro-Survival Protein Mcl-1.  |  Beekman, AM. and Howell, LA. 2016. ChemMedChem. 11: 802-13. PMID: 26696548
2. MCL-1 is a prognostic indicator and drug target in breast cancer.  |  Campbell, KJ., et al. 2018. Cell Death Dis. 9: 19. PMID: 29339815
3. Co-expression of Mcl-1 and Bak induces mitochondrial swelling.  |  Wang, Y., et al. 2020. Biochem Biophys Res Commun. 527: 866-873. PMID: 32430171
4. Epigenetic modulation of FBW7/Mcl-1 pathway for lung cancer therapy.  |  Kim, MJ., et al. 2021. Cancer Biol Ther. 22: 55-65. PMID: 33336620
5. Targeting MCL-1 dysregulates cell metabolism and leukemia-stroma interactions and resensitizes acute myeloid leukemia to BCL-2 inhibition.  |  Carter, BZ., et al. 2022. Haematologica. 107: 58-76. PMID: 33353284
6. Phosphatase PP2A enhances MCL-1 protein half-life in multiple myeloma cells.  |  Slomp, A., et al. 2021. Cell Death Dis. 12: 229. PMID: 33658484
7. The Role of Mcl-1 in Embryonic Neural Precursor Cell Apoptosis.  |  Flemmer, RT., et al. 2021. Front Cell Dev Biol. 9: 659531. PMID: 33959612
8. MiRNA-16-1 Suppresses Mcl-1 and Bcl-2 and Sensitizes Chronic Lymphocytic Leukemia Cells to BH3 Mimetic ABT-199.  |  Ashofteh, N., et al. 2022. Cell J. 24: 473-480. PMID: 36093807
9. Decoding the mechanism behind MCL-1 inhibitors: A pathway to understanding MCL-1 protein stability.  |  Tantawy, SI., et al. 2023. Oncotarget. 14: 653-655. PMID: 37343064
10. Development of an MCL-1-related prognostic signature and inhibitors screening for glioblastoma.  |  Zhang, A., et al. 2023. Front Pharmacol. 14: 1162540. PMID: 37538176

The preferred Mcl-1 antibody is Mcl-1 Antibody (22): sc-12756.