Mcl-1 Antibody (G-7): sc-74437
- Mcl-1 Antibody (G-7) is a mouse monoclonal IgG2a κ, cited in 14 publications, provided at 200 µg/ml
- raised against amino acids 1-260 of Mcl-1 of human origin
- Anti-Mcl-1 Antibody (G-7) is recommended for detection of Mcl-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Mcl-1 Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Mcl-1 Antibody (G-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Mcl-1 Antibody (G-7) (see ordering information below).
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SEE ALSO...
Mcl-1 Antibody (G-7) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 1-260 of human Mcl-1, suitable for detecting Mcl-1 proteins from mouse, rat, and human origins via western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. Mcl-1 (Myeloid cell leukemia 1) is a critical anti-apoptotic protein within the B-cell lymphoma 2 (Bcl-2) family, playing a vital role in cell survival by inhibiting apoptosis through interactions with several key proteins. Mcl-1 monoclonal antibody (G-7) binds to pro-apoptotic members such as Bak (Bcl-2 antagonist killer) and Bax (Bcl-2-associated X protein), sequestering them and preventing mitochondrial outer membrane permeabilization and cytochrome c release, which are essential steps in the intrinsic apoptotic pathway. Anti-Mcl-1 antibody (G-7) also interacts with BH3-only proteins like Bim (Bcl-2-interacting mediator of cell death), NOXA, and PUMA, which can bind to Mcl-1 and neutralize anti-apoptotic function, thereby promoting apoptosis when necessary. Stability and expression of Mcl-1 are regulated by interactions with E3 ubiquitin ligases such as Mcl-1 ubiquitin ligase E3 (MULE) and β-TrCP, targeting for ubiquitination and proteasomal degradation. Glycogen synthase kinase 3 (GSK3) phosphorylates Mcl-1, facilitating recognition by these ubiquitin ligases. Additionally, tumor suppressor p53 can influence Mcl-1 levels by promoting expression of pro-apoptotic proteins that antagonize Mcl-1 function. This intricate network of interactions involving Mcl-1 is crucial for maintaining cellular homeostasis, and dysregulation can lead to diseases such as cancer, where overexpression of Mcl-1 contributes to tumor progression and resistance to chemotherapy. Mcl-1 (G-7) antibody serves as an invaluable tool for researchers investigating molecular mechanisms of apoptosis, protein-protein interactions involving Mcl-1, and implications in human diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mcl-1 Antibody (G-7) | sc-74437 | 200 µg/ml | $322.00 | |||
Mcl-1 Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-525708 | 200 µg Ab; 10 µg BP | $361.00 | |||
Mcl-1 Antibody (G-7): m-IgG2a BP-HRP Bundle | sc-546370 | 200 µg Ab; 10 µg BP | $361.00 | |||
Mcl-1 Antibody (G-7) AC | sc-74437 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Mcl-1 Antibody (G-7) HRP | sc-74437 HRP | 200 µg/ml | $322.00 | |||
Mcl-1 Antibody (G-7) FITC | sc-74437 FITC | 200 µg/ml | $336.00 | |||
Mcl-1 Antibody (G-7) PE | sc-74437 PE | 200 µg/ml | $349.00 | |||
Mcl-1 Antibody (G-7) Alexa Fluor® 488 | sc-74437 AF488 | 200 µg/ml | $364.00 | |||
Mcl-1 Antibody (G-7) Alexa Fluor® 546 | sc-74437 AF546 | 200 µg/ml | $364.00 | |||
Mcl-1 Antibody (G-7) Alexa Fluor® 594 | sc-74437 AF594 | 200 µg/ml | $364.00 | |||
Mcl-1 Antibody (G-7) Alexa Fluor® 647 | sc-74437 AF647 | 200 µg/ml | $364.00 | |||
Mcl-1 Antibody (G-7) Alexa Fluor® 680 | sc-74437 AF680 | 200 µg/ml | $364.00 | |||
Mcl-1 Antibody (G-7) Alexa Fluor® 790 | sc-74437 AF790 | 200 µg/ml | $364.00 |