Mcl-1 Antibody (B-6): sc-74436

Mcl-1 Antibody (B-6) is a mouse monoclonal IgG2a kappa light chain antibody raised against amino acids 1-260 of human Mcl-1, designed for detecting Mcl-1 proteins from mouse, rat, and human origins via western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. Mcl-1, also called Myeloid cell leukemia-1, Mcl1, and Gm52627, is a crucial anti-apoptotic protein within the B-cell lymphoma 2 (Bcl-2) family, playing a vital role in cell survival by inhibiting apoptosis through binding to pro-apoptotic proteins such as Bak and Bax, thereby preventing mitochondrial outer membrane permeabilization and cytochrome c release. Notably, Mcl-1 undergoes several post-translational modifications, including phosphorylation, ubiquitination, and proteolytic cleavage, which are essential for regulating stability, localization, and function. For instance, phosphorylation by glycogen synthase kinase 3 (GSK3) targets Mcl-1 for ubiquitination and subsequent proteasomal degradation, allowing cells to swiftly modulate apoptosis in response to stress signals. These modifications are significant because dysregulation of Mcl-1′s post-translational modifications can lead to aberrant cell survival and has been implicated in the development of various cancers, where overexpression of Mcl-1 contributes to tumor progression and resistance to chemotherapy. Understanding the post-translational regulation of Mcl-1 is therefore critical for therapeutic interventions targeting apoptosis pathways. Anti-Mcl-1 antibody (B-6) serves as an invaluable tool for researchers studying the molecular mechanisms of cell death and survival, the role of Mcl-1 in disease, and the impact of post-translational modifications on cellular functions.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mcl-1 Antibody (B-6) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Mcl-1 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mcl-1 Antibody (B-6) Specifications

Alternate Product Name: MCL1

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-260

Epitope Target: Mcl-1

Epitope Species: human

Product Reactivity/Detection: Mcl-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Mcl-1 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Mcl-1 antibody is Mcl-1 Antibody (22): sc-12756.