MBP-probe Antibody (TVG MBP 3): sc-53367

MBP-probe Antibody (TVG MBP 3) is a mouse monoclonal IgM antibody that detects maltose binding protein (MBP) of non-species reactive (NSR) by western blotting (WB) and immunoprecipitation (IP). Anti-MBP antibody (TVG MBP 3) is designed to facilitate the study of MBP, a 370 amino acid protein derived from the malE gene of Escherichia coli, which plays a crucial role in the transport of maltose and maltodextrins across the bacterial cell membrane. MBP′s ability to bind maltose with high affinity is essential for bacterial metabolism, as this binding allows for efficient uptake of these sugars, which serve as vital energy sources. MBP′s significance extends beyond transport function; MBP is widely utilized in molecular biology as a fusion tag for recombinant protein purification. By leveraging MBP′s unique properties, researchers can easily isolate target proteins through affinity chromatography, enhancing protein purification efficiency. MBP-probe antibody (TVG MBP 3) serves as an invaluable tool for scientists exploring the functional dynamics of MBP and associated fusion proteins in various experimental contexts.

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MBP-probe Antibody (TVG MBP 3) References:

1. Insights into the conformational equilibria of maltose-binding protein by analysis of high affinity mutants.  |  Telmer, PG. and Shilton, BH. 2003. J Biol Chem. 278: 34555-67. PMID: 12794084
2. The energetic cost of domain reorientation in maltose-binding protein as studied by NMR and fluorescence spectroscopy.  |  Millet, O., et al. 2003. Proc Natl Acad Sci U S A. 100: 12700-5. PMID: 14530390
3. Both maltose-binding protein and ATP are required for nucleotide-binding domain closure in the intact maltose ABC transporter.  |  Orelle, C., et al. 2008. Proc Natl Acad Sci U S A. 105: 12837-42. PMID: 18725638
4. Immunogenicity of recombinant maltose-binding protein (MBP)-gonadotropin releasing hormone I (GnRH-I).  |  Fang, F., et al. 2010. Syst Biol Reprod Med. 56: 478-86. PMID: 20849227
5. Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein.  |  Dälken, B., et al. 2010. PLoS One. 5: e14404. PMID: 21203542
6. Accessing a hidden conformation of the maltose binding protein using accelerated molecular dynamics.  |  Bucher, D., et al. 2011. PLoS Comput Biol. 7: e1002034. PMID: 21533070
7. MUC1 and maltose‑binding protein recombinant fusion protein combined with Bacillus Calmette‑Guerin induces MUC1‑specific and nonspecific anti‑tumor immunity in mice.  |  Fang, F., et al. 2014. Mol Med Rep. 10: 1056-64. PMID: 24912810
8. Full engagement of liganded maltose-binding protein stabilizes a semi-open ATP-binding cassette dimer in the maltose transporter.  |  Alvarez, FJ., et al. 2015. Mol Microbiol. 98: 878-94. PMID: 26268698
9. The combination of maltose-binding protein and BCG-induced Th1 activation is involved in TLR2/9-mediated upregulation of MyD88-TRAF6 and TLR4-mediated downregulation of TRIF-TRAF3.  |  Liu, G., et al. 2018. Cell Immunol. 325: 56-63. PMID: 29452695
10. Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin.  |  Sachdev, D. and Chirgwin, JM. 1998. Protein Expr Purif. 12: 122-32. PMID: 9473466

The preferred MBP-probe antibody is MBP-probe Antibody (R29.6): sc-13564.