MBP-probe Antibody (R29.6): sc-13564

MBP-probe Antibody (R29.6) is a mouse monoclonal IgG1 kappa light chain antibody that detects MBP-probe protein by western blotting (WB) and immunoprecipitation (IP). Anti-MBP-probe antibody (R29.6) is available in both non-conjugated and various conjugated forms, including agarose, phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. MBP-probe protein, derived from the malE gene of Escherichia coli, consists of 370 amino acids and plays a crucial role in facilitating the purification of hybrid fusion proteins. This is particularly important in molecular biology, as MBP domain allows for efficient isolation of target proteins through affinity chromatography using amylose resin. Once MBP-fusion protein is bound to the resin, separation from the target protein occurs by cleavage with coagulation factor Xa or elution with excess free maltose, enabling researchers to obtain high-purity proteins for further analysis. Visualization of these proteins through techniques such as western blotting (WB) and immunoprecipitation (IP) enhances MBP-probe (R29.6) antibody′s utility in various applications, making this tool essential for researchers working with fusion proteins in both prokaryotic and eukaryotic systems.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MBP-probe Antibody (R29.6) References:

1. Purification of proteins fused to maltose-binding protein.  |  Lebendiker, M. and Danieli, T. 2011. Methods Mol Biol. 681: 281-93. PMID: 20978971
2. Maltose-binding protein is a potential carrier for oral immunizations.  |  Bellot, P., et al. 2013. Vet Immunol Immunopathol. 152: 101-8. PMID: 23078905
3. The ability to enhance the solubility of its fusion partners is an intrinsic property of maltose-binding protein but their folding is either spontaneous or chaperone-mediated.  |  Raran-Kurussi, S. and Waugh, DS. 2012. PLoS One. 7: e49589. PMID: 23166722
4. Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems.  |  Reuten, R., et al. 2016. PLoS One. 11: e0152386. PMID: 27029048
5. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein.  |  di Guan, C., et al. 1988. Gene. 67: 21-30. PMID: 2843437
6. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.  |  Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
7. An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein.  |  Maina, CV., et al. 1988. Gene. 74: 365-73. PMID: 3073105
8. A useful epitope tag derived from maltose binding protein.  |  Lénon, M., et al. 2021. Protein Sci. 30: 1235-1246. PMID: 33896065
9. Peptide ligand interaction with maltose-binding protein tagged to the calcitonin gene-related peptide receptor: The inhibitory role of receptor N-glycosylation.  |  Lee, S. 2022. Peptides. 150: 170735. PMID: 35007660
10. Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12.  |  Duplay, P., et al. 1984. J Biol Chem. 259: 10606-13. PMID: 6088507
11. A multifunctional prokaryotic protein expression system: overproduction, affinity purification, and selective detection.  |  Kroll, DJ., et al. 1993. DNA Cell Biol. 12: 441-53. PMID: 7686011

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MBP-probe Antibody (R29.6) has 27 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MBP-probe Antibody (R29.6) Specifications

Alternate Product Name: Maltose Binding Protein

Product Clone Name: R29.6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MBP-probe

Epitope Species:

Product Reactivity/Detection: MBP fusion proteins

Species Reactivity/Detection: N/A

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for MBP-probe Antibody (R29.6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin