MBP Antibody (F-6): sc-271524

MBP Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects MBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MBP Antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Myelin basic protein (MBP) plays a crucial role as the primary extrinsic membrane protein of central nervous system myelin, essential for the formation and maintenance of the myelin sheath that insulates nerve fibers, thereby facilitating efficient signal transmission. MBP (F-6) antibody enables researchers to study the phosphorylation of MBP at Threonine 125, a complex regulatory mechanism that significantly influences myelin sheath stability. This phosphorylation is modulated by mitogen-activated protein kinases during myelinogenesis and in demyelinating diseases such as multiple sclerosis. Notably, MBP phosphorylation responds to high-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus, but not to low-frequency stimulation. During heightened neuronal activity, calcium influx activates axonal nitric oxide synthase, leading to the production of intercellular messengers like nitric oxide and superoxide, which regulate MBP phosphorylation through MAPK pathways. This intricate regulation underscores MBP′s importance in both normal and pathological states of the nervous system, making anti-MBP antibody (F-6) a valuable tool for research in neurobiology and related fields.

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MBP Antibody (F-6) References:

1. Regulation of myelin basic protein phosphorylation by mitogen-activated protein kinase during increased action potential firing in the hippocampus.  |  Atkins, CM., et al. 1999. J Neurochem. 73: 1090-7. PMID: 10461899
2. Encephalitogenic potential of the myelin basic protein peptide (amino acids 83-99) in multiple sclerosis: results of a phase II clinical trial with an altered peptide ligand.  |  Bielekova, B., et al. 2000. Nat Med. 6: 1167-75. PMID: 11017150
3. Expression of myelin basic protein (MBP) epitopes in human non-neural cells revealed by two anti-MBP IgM monoclonal antibodies.  |  Chignola, R., et al. 2000. Clin Exp Immunol. 122: 429-36. PMID: 11122251
4. Relationship of urinary myelin basic protein-like material with cranial magnetic resonance imaging in advanced multiple sclerosis.  |  Whitaker, JN., et al. 2001. Arch Neurol. 58: 49-54. PMID: 11176936
5. The effects of pregnancy on myelin basic protein-induced experimental autoimmune encephalomyelitis in Lewis rats: suppression of clinical disease, modulation of cytokine expression in the spinal cord inflammatory infiltrate and suppression of lymphocyte proliferation by pregnancy sera.  |  Harness, J. and McCombe, PA. 2001. Am J Reprod Immunol. 46: 405-12. PMID: 11775010
6. Myelin basic protein (MBP) displays significant homologies with gag core proteins of HTLV retroviruses.  |  Liquori, AM. 1991. J Theor Biol. 148: 279-81. PMID: 1708065
7. Structure and function of the proline-rich region of myelin basic protein.  |  Fraser, PE. and Deber, CM. 1985. Biochemistry. 24: 4593-8. PMID: 2415158
8. Identification of an antigenic determinant within the phylogenetically conserved triprolyl region of myelin basic protein.  |  Potter, NT., et al. 1986. J Immunol. 136: 516-20. PMID: 2416809
9. The glycosylation of human myelin basic protein at threonines 95 and 98 occurs sequentially.  |  Persaud, R., et al. 1988. Biochim Biophys Acta. 966: 357-61. PMID: 2458139
10. Docking and molecular dynamics simulations of the Fyn-SH3 domain with free and phospholipid bilayer-associated 18.5-kDa myelin basic protein (MBP)-Insights into a noncanonical and fuzzy interaction.  |  Bessonov, K., et al. 2017. Proteins. 85: 1336-1350. PMID: 28380689
11. Design and Synthesis of Non-Peptide Mimetics Mapping the Immunodominant Myelin Basic Protein (MBP83-96) Epitope to Function as T-Cell Receptor Antagonists.  |  Yannakakis, MP., et al. 2017. Int J Mol Sci. 18: PMID: 28594344
12. Identification of a mitogen-activated protein kinase site in human myelin basic protein in situ.  |  Yon, M., et al. 1996. J Neuroimmunol. 65: 55-9. PMID: 8642064

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MBP Antibody (F-6) has 63 citations in a variety of scientific publications.

Primary antibodies like Anti-MBP Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MBP Antibody (F-6) Specifications

Alternate Product Name: myelin basic protein

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 196-237

Epitope Target: MBP

Epitope Species: human

Product Reactivity/Detection: MBP isoforms 1, 3, 4, 5 and 6, (also designated Golli-MBP1, MBP1, MBP2, MBP3 and MBP4)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 63 citations in various scientific publications

Available Conjugates/Forms for MBP Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin