MBNL3 CRISPR/Cas9 KO Plasmid (h): sc-406573
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MBNL3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MBNL3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MBNL3 Antibody (5A11): sc-136168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MBNL3 CRISPR/Cas9 KO Plasmid (h) | sc-406573 | 20 µg | $397.00 |
Overview
MBNL3 (muscleblind-like splicing regulator 3) is an RNA-binding protein that recognizes YGCY motifs in pre-mRNAs to control alternative splicing, mRNA stability, and localization. It participates in post-transcriptional gene regulation programs that shape cell-state transitions, including differentiation-associated splicing decisions and tissue-specific transcript isoform selection. MBNL family proteins are central nodes in RNA processing networks linked to cytoskeletal organization, signaling output, and metabolic remodeling through isoform-dependent regulation. Dysregulation of MBNL-mediated splicing has been implicated in RNA toxicity–driven neuromuscular disease mechanisms and broader transcriptome-wide splicing perturbations observed in developmental and cancer-associated contexts.
MBNL3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MBNL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MBNL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MBNL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MBNL3 protein expression.
This CRISPR knockout system enables efficient generation of MBNL3-deficient cell models for investigation of MBNL3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MBNL3 exon(s) critical for MBNL3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MBNL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MBNL3 CRISPR/Cas9 KO Plasmid (h) and MBNL3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MBNL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MBNL3 HDR Plasmid (h) and MBNL3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MBNL3 homology arms to support homology-directed repair at defined MBNL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.