MBLAC2 Antibody (E-7): sc-398153. Western blot analysis of MBLAC2 expression in non-transfected: sc-117752 (A) and human MBLAC2 transfected: sc-115432 (B) 293T whole cell lysates and mouse brain (C) and human brain (D) tissue extracts.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MBLAC2 Antibody (E-7): sc-398153. Western blot analysis of MBLAC2 expression in non-transfected: sc-117752 (A) and human MBLAC2 transfected: sc-115432 (B) 293T whole cell lysates and mouse brain (C) and human brain (D) tissue extracts.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
MBLAC2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MBLAC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MBLAC2 Antibody (E-7): sc-398153