MBD2/3 Antibody (B-12): sc-271522
- MBD2/3 Antibody (B-12) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 242-291 mapping at the C-terminus of MBD3 of human origin
- recommended for detection of MBD2 and MBD3 of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for MBD2/3 Antibody (B-12) for WB applications. These reagents are now offered in bundles with MBD2/3 Antibody (B-12) (see ordering information below).
MBD2/3 Antibody (B-12) is a mouse monoclonal IgG2a antibody that detects MBD2/3 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MBD2/3 plays a crucial role in the regulation of gene expression by binding to methylated DNA, particularly at methyl-CpG sites, which are often associated with transcriptional repression. This interaction is vital for maintaining cellular homeostasis and regulating developmental processes, as aberrations in DNA methylation can lead to various diseases, including cancer. MBD2 forms complexes with histone deacetylases, which further contribute to the repression of transcription in methylated genes. MBD2/3′s ability to modulate gene expression highlights its importance in epigenetic regulation and underscores anti-MBD2/3 antibody (B-12)′s relevance in research focused on understanding gene regulation mechanisms and their implications in health and disease.
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MBD2/3 Antibody (B-12) References:
- Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes. | Hendrich, B., et al. 1999. Mamm Genome. 10: 906-12. PMID: 10441743
- MBD2-MBD3 complex binds to hemi-methylated DNA and forms a complex containing DNMT1 at the replication foci in late S phase. | Tatematsu, KI., et al. 2000. Genes Cells. 5: 677-88. PMID: 10947852
- MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing. | Jin, SG., et al. 2005. J Biol Chem. 280: 12700-9. PMID: 15701600
- Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences. | Günther, K., et al. 2013. Nucleic Acids Res. 41: 3010-21. PMID: 23361464
- Reduced mRNA expression levels of MBD2 and MBD3 in gastric carcinogenesis. | Pontes, TB., et al. 2014. Tumour Biol. 35: 3447-53. PMID: 24338710
- Structural analyses reveal that MBD3 is a methylated CG binder. | Liu, K., et al. 2019. FEBS J. 286: 3240-3254. PMID: 30980593
- Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells. | Dai, Y., et al. 2020. Am J Transl Res. 12: 8147-8161. PMID: 33437388
- MBD2 acts as a repressor to maintain the homeostasis of the Th1 program in type 1 diabetes by regulating the STAT1-IFN-γ axis. | Yue, T., et al. 2022. Cell Death Differ. 29: 218-229. PMID: 34420035
- MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma. | Chen, Z., et al. 2022. Front Genet. 13: 959059. PMID: 36303542
- MBD3 promotes epithelial-mesenchymal transition in gastric cancer cells by upregulating ACTG1 via the PI3K/AKT pathway. | Wang, H., et al. 2024. Biol Proced Online. 26: 1. PMID: 38178023
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MBD2/3 Antibody (B-12) | sc-271522 | 200 µg/ml | $322.00 | |||
MBD2/3 Antibody (B-12): m-IgG Fc BP-HRP Bundle | sc-540243 | 200 µg Ab; 10 µg BP | $361.00 | |||
MBD2/3 Antibody (B-12): m-IgG2a BP-HRP Bundle | sc-546876 | 200 µg Ab; 10 µg BP | $361.00 |