MBD3 Antibody (F-11): sc-166319
- MBD3 Antibody (F-11) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 234-267 near the C-terminus of MBD3 of human origin
- Anti-MBD3 Antibody (F-11) is recommended for detection of MBD3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including porcine
- Anti-MBD3 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP is the preferred secondary detection reagent for MBD3 Antibody (F-11) for WB applications. This reagent is now offered in a bundle with MBD3 Antibody (F-11) (see ordering information below).
QUICK LINKS
MBD3 Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects MBD3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MBD3 Antibody (F-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MBD3 plays a crucial role in gene expression regulation through DNA methylation involvement, essential for maintaining cellular identity and function. MBD3 functions as part of the methyl-CpG binding protein family, recognizing and binding to methylated cytosine residues within CpG dinucleotides, influencing transcriptional repression. This interaction enables gene silencing in specific cell types, contributing to development, differentiation, and genomic stability. MBD3 interacts with other methyl-CpG binding protein family members, including MBD1, MBD2, and MeCP2, forming complexes that mediate transcriptional repression in response to DNA methylation. MBD proteins show high expression in somatic tissues, highlighting their significance in gene expression regulation across various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MBD3 Antibody (F-11) References:
- Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes. | Hendrich, B., et al. 1999. Mamm Genome. 10: 906-12. PMID: 10441743
- Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms. | Fujita, N., et al. 1999. Mol Cell Biol. 19: 6415-26. PMID: 10454587
- MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex. | Ng, HH., et al. 1999. Nat Genet. 23: 58-61. PMID: 10471499
- The thymine glycosylase MBD4 can bind to the product of deamination at methylated CpG sites. | Hendrich, B., et al. 1999. Nature. 401: 301-4. PMID: 10499592
- Solution structure of the methyl-CpG-binding domain of the methylation-dependent transcriptional repressor MBD1. | Ohki, I., et al. 1999. EMBO J. 18: 6653-61. PMID: 10581239
- DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein. | Boyes, J. and Bird, A. 1991. Cell. 64: 1123-34. PMID: 2004419
- Structural analyses reveal that MBD3 is a methylated CG binder. | Liu, K., et al. 2019. FEBS J. 286: 3240-3254. PMID: 30980593
- Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells. | Dai, Y., et al. 2020. Am J Transl Res. 12: 8147-8161. PMID: 33437388
- MBD3 promotes hepatocellular carcinoma progression and metastasis through negative regulation of tumour suppressor TFPI2. | Yan, W., et al. 2022. Br J Cancer. 127: 612-623. PMID: 35501390
- MBD3 promotes epithelial-mesenchymal transition in gastric cancer cells by upregulating ACTG1 via the PI3K/AKT pathway. | Wang, H., et al. 2024. Biol Proced Online. 26: 1. PMID: 38178023
- Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. | Nan, X., et al. 1998. Nature. 393: 386-9. PMID: 9620804
- Identification and characterization of a family of mammalian methyl-CpG binding proteins. | Hendrich, B. and Bird, A. 1998. Mol Cell Biol. 18: 6538-47. PMID: 9774669
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MBD3 Antibody (F-11) | sc-166319 | 200 µg/ml | $322.00 | |||
MBD3 Antibody (F-11): m-IgG2a BP-HRP Bundle | sc-545927 | 200 µg Ab; 10 µg BP | $361.00 | |||
MBD3 Antibody (F-11) AC | sc-166319 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MBD3 Antibody (F-11) HRP | sc-166319 HRP | 200 µg/ml | $322.00 | |||
MBD3 Antibody (F-11) FITC | sc-166319 FITC | 200 µg/ml | $336.00 | |||
MBD3 Antibody (F-11) PE | sc-166319 PE | 200 µg/ml | $349.00 | |||
MBD3 Antibody (F-11) Alexa Fluor® 488 | sc-166319 AF488 | 200 µg/ml | $364.00 | |||
MBD3 Antibody (F-11) Alexa Fluor® 546 | sc-166319 AF546 | 200 µg/ml | $364.00 | |||
MBD3 Antibody (F-11) Alexa Fluor® 594 | sc-166319 AF594 | 200 µg/ml | $364.00 | |||
MBD3 Antibody (F-11) Alexa Fluor® 647 | sc-166319 AF647 | 200 µg/ml | $364.00 | |||
MBD3 Antibody (F-11) Alexa Fluor® 680 | sc-166319 AF680 | 200 µg/ml | $364.00 | |||
MBD3 Antibody (F-11) Alexa Fluor® 790 | sc-166319 AF790 | 200 µg/ml | $364.00 | |||
MBD3 (F-11) Neutralizing Peptide | sc-166319 P | 100 µg/0.5 ml | $69.00 |