MBD1 Antibody (B-5): sc-25261

MBD1 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects MBD1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MBD1 Antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MBD1 protein plays a crucial role in gene expression regulation through interaction with methylated DNA, specifically binding to methyl-CpG sites found in gene promoter regions. This binding mediates transcriptional repression, a process vital for normal cellular function and development. MBD1′s recognition and binding to methylated regions enables recruitment of other proteins, such as histone deacetylases, to form repressor complexes that silence gene expression. MBD1′s importance extends beyond transcriptional regulation, involving processes such as genomic stability and cellular identity maintenance. Since MBD proteins show highest expression in somatic tissues, anti-MBD1 antibody (B-5) serves as a valuable tool for researchers studying epigenetic regulation and implications in various biological contexts, including development, differentiation, and disease.

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MBD1 Antibody (B-5) References:

1. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes.  |  Hendrich, B., et al. 1999. Mamm Genome. 10: 906-12. PMID: 10441743
2. Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms.  |  Fujita, N., et al. 1999. Mol Cell Biol. 19: 6415-26. PMID: 10454587
3. MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex.  |  Ng, HH., et al. 1999. Nat Genet. 23: 58-61. PMID: 10471499
4. The thymine glycosylase MBD4 can bind to the product of deamination at methylated CpG sites.  |  Hendrich, B., et al. 1999. Nature. 401: 301-4. PMID: 10499592
5. Solution structure of the methyl-CpG-binding domain of the methylation-dependent transcriptional repressor MBD1.  |  Ohki, I., et al. 1999. EMBO J. 18: 6653-61. PMID: 10581239
6. Regulation of transcription and chromatin by methyl-CpG binding protein MBD1.  |  Nakao, M., et al. 2001. Brain Dev. 23 Suppl 1: S174-6. PMID: 11738867
7. DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein.  |  Boyes, J. and Bird, A. 1991. Cell. 64: 1123-34. PMID: 2004419
8. MBD1 is an Epigenetic Regulator of KEAP1 in Pancreatic Cancer.  |  Zhang, B., et al. 2016. Curr Mol Med. 16: 404-11. PMID: 26980696
9. Methyl-CpG-Binding Protein MBD1 Regulates Neuronal Lineage Commitment through Maintaining Adult Neural Stem Cell Identity.  |  Jobe, EM., et al. 2017. J Neurosci. 37: 523-536. PMID: 28100736
10. MBD1 protects replication fork stability by recruiting PARP1 and controlling transcription-replication conflicts.  |  Yu, G., et al. 2024. Cancer Gene Ther. 31: 94-107. PMID: 37949945
11. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex.  |  Nan, X., et al. 1998. Nature. 393: 386-9. PMID: 9620804
12. Identification and characterization of a family of mammalian methyl-CpG binding proteins.  |  Hendrich, B. and Bird, A. 1998. Mol Cell Biol. 18: 6538-47. PMID: 9774669

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MBD1 Antibody (B-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-MBD1 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MBD1 Antibody (B-5) Specifications

Alternate Product Name: MBD1

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 383-636

Epitope Target: MBD1

Epitope Species: mouse

Product Reactivity/Detection: MBD1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for MBD1 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin