Max Antibody (H-2): sc-8011
- Max Antibody (H-2) is a mouse monoclonal IgG1 κ Max antibody, cited in 29 publications, provided at 200 µg/ml
- raised against amino acids 28-151 mapping at the C-terminus of Max of human origin
- Max Antibody (H-2) is recommended for detection of Max p21 and Max p22 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
- Anti-Max Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-8011 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Max Antibody (H-2) for WB and IHC(P) applications. These reagents are now offered in bundles with Max Antibody (H-2) (see ordering information below).
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Max Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Max protein in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Max antibody (H-2) comes in non-conjugated forms and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. Max protein functions as a key regulator of gene expression within transcription factor complexes, notably forming heterodimers with Myc proteins to enhance DNA binding and transcriptional activation. Max-Myc heterodimers bind to E-box sequence CACGTG more strongly than Max homodimers, influencing target gene expression. Max′s stable presence in cells, unlike highly regulated Myc, ensures proper function of transcriptional networks. Max interactions with proteins like Mad and Mxi1 can inhibit Max-Myc activity, highlighting complex regulatory mechanisms in cellular behavior and oncogenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Max Antibody (H-2) References:
- Determination of binding constant of transcription factor myc-max/max-max and E-box DNA: the effect of inhibitors on the binding. | Park, S., et al. 2004. Biochim Biophys Acta. 1670: 217-28. PMID: 14980448
- Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants. | Mukherjee, B., et al. 1992. Genes Dev. 6: 1480-92. PMID: 1644290
- Association of Myn, the murine homolog of max, with c-Myc stimulates methylation-sensitive DNA binding and ras cotransformation. | Prendergast, GC., et al. 1991. Cell. 65: 395-407. PMID: 1840505
- Intracellular leucine zipper interactions suggest c-Myc hetero-oligomerization. | Dang, CV., et al. 1991. Mol Cell Biol. 11: 954-62. PMID: 1990293
- Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. | Blackwood, EM. and Eisenman, RN. 1991. Science. 251: 1211-7. PMID: 2006410
- Small-molecule modulators of c-Myc/Max and Max/Max interactions. | Berg, T. 2011. Curr Top Microbiol Immunol. 348: 139-49. PMID: 20680803
- Transcriptional regulation by dimerization: two sides to an incestuous relationship. | Jones, N. 1990. Cell. 61: 9-11. PMID: 2180585
- Unexpected link between MAX and meiotic onset. | Okuda, A. and Suzuki, A. 2016. Cell Cycle. 15: 2235-6. PMID: 27267300
- Distinct DNA binding preferences for the c-Myc/Max and Max/Max dimers. | Solomon, DL., et al. 1993. Nucleic Acids Res. 21: 5372-6. PMID: 8265351
- Mad: a heterodimeric partner for Max that antagonizes Myc transcriptional activity. | Ayer, DE., et al. 1993. Cell. 72: 211-22. PMID: 8425218
- Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites. | Zervos, AS., et al. 1993. Cell. 72: 223-32. PMID: 8425219
- Oncogenic activity of the c-Myc protein requires dimerization with Max. | Amati, B., et al. 1993. Cell. 72: 233-45. PMID: 8425220
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Max Antibody (H-2) | sc-8011 | 200 µg/ml | $322.00 | |||
Max Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-528200 | 200 µg Ab; 10 µg BP | $361.00 | |||
Max Antibody (H-2): m-IgGκ BP-HRP Bundle | sc-520522 | 200 µg Ab, 40 µg BP | $361.00 | |||
Max Antibody (H-2): m-IgG1 BP-HRP Bundle | sc-542812 | 200 µg Ab; 20 µg BP | $361.00 | |||
Max Antibody (H-2) X | sc-8011 X | 200 µg/0.1 ml | $322.00 | |||
Max Antibody (H-2) AC | sc-8011 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Max Antibody (H-2) HRP | sc-8011 HRP | 200 µg/ml | $322.00 | |||
Max Antibody (H-2) FITC | sc-8011 FITC | 200 µg/ml | $336.00 | |||
Max Antibody (H-2) PE | sc-8011 PE | 200 µg/ml | $349.00 | |||
Max Antibody (H-2) Alexa Fluor® 488 | sc-8011 AF488 | 200 µg/ml | $364.00 | |||
Max Antibody (H-2) Alexa Fluor® 546 | sc-8011 AF546 | 200 µg/ml | $364.00 | |||
Max Antibody (H-2) Alexa Fluor® 594 | sc-8011 AF594 | 200 µg/ml | $364.00 | |||
Max Antibody (H-2) Alexa Fluor® 647 | sc-8011 AF647 | 200 µg/ml | $364.00 | |||
Max Antibody (H-2) Alexa Fluor® 680 | sc-8011 AF680 | 200 µg/ml | $364.00 | |||
Max Antibody (H-2) Alexa Fluor® 790 | sc-8011 AF790 | 200 µg/ml | $364.00 |