Matrilin-2 CRISPR/Cas9 KO Plasmid (h): sc-404852
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Matrilin-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Matrilin-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Matrilin-2 Antibody (2B8): sc-293397
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Matrilin-2 CRISPR/Cas9 KO Plasmid (h) | sc-404852 | 20 µg | $397.00 |
Overview
MATN2 encodes matrilin-2, a multidomain extracellular matrix (ECM) adapter glycoprotein that participates in assembling and stabilizing filamentous networks by bridging collagens, proteoglycans, and other matrix constituents. Matrilin-2 supports tissue architecture and cell–matrix communication that influence adhesion, migration, and mechanotransduction, processes closely linked to ECM remodeling programs. Through its roles in matrix organization, MATN2 is relevant to studies of musculoskeletal and connective tissue biology and broader contexts where ECM composition shapes inflammation and wound repair. Dysregulated ECM homeostasis involving MATN2 has been associated with pathological remodeling and fibrosis-like phenotypes in multiple tissues, motivating mechanistic investigation in human cell models.
Matrilin-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MATN2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MATN2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MATN2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Matrilin-2 protein expression.
This CRISPR knockout system enables efficient generation of MATN2-deficient cell models for investigation of Matrilin-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MATN2 exon(s) critical for Matrilin-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MATN2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Matrilin-2 CRISPR/Cas9 KO Plasmid (h) and Matrilin-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MATN2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Matrilin-2 HDR Plasmid (h) and Matrilin-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MATN2 homology arms to support homology-directed repair at defined MATN2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.