Mat1 Antibody (F-6): sc-13142

Mat1 Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mat1 protein of mouse, rat, and human origin through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). Mat1 (F-6) antibody is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. Mat1 plays a crucial role in the regulation of cyclin-dependent kinases (Cdks), which are essential for cell cycle progression. Mat1 functions as a key component of the cyclin H-Cdk7 complex, which is vital for the activation of Cdks through phosphorylation. This interaction is critical because the activation of Cdks is necessary for the transition between different phases of the cell cycle, ensuring proper cell division and function. Mat1 not only facilitates the assembly of the cyclin H-Cdk7 complex but also enhances kinase activity, thereby influencing the phosphorylation of target proteins such as Cdk2 and RNA polymerase II. Understanding Mat1′s role in Cdk activation provides insights into mechanisms governing cell cycle regulation and implications in various biological processes and diseases.

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Mat1 Antibody (F-6) References:

1. Corepressor MMTR/DMAP1 is involved in both histone deacetylase 1- and TFIIH-mediated transcriptional repression.  |  Kang, BG., et al. 2007. Mol Cell Biol. 27: 3578-88. PMID: 17371848
2. Unexpected placement of the MAT1-1-2 gene in the MAT1-2 idiomorph of Thielaviopsis.  |  Wilken, PM., et al. 2018. Fungal Genet Biol. 113: 32-41. PMID: 29409964
3. MAT1 correlates with molecular subtypes and predicts poor survival in breast cancer.  |  Xu, H., et al. 2018. Chin J Cancer Res. 30: 351-363. PMID: 30046229
4. Chromosomal instability-associated MAT1 lncRNA insulates MLL1-guided histone methylation and accelerates tumorigenesis.  |  Pan, H., et al. 2022. Cell Rep. 41: 111829. PMID: 36516779
5. Alternative mechanisms of CAK assembly require an assembly factor or an activating kinase.  |  Fisher, RP., et al. 1995. Cell. 83: 47-57. PMID: 7553872
6. Activation of cyclin-dependent kinase 4 (cdk4) by mouse MO15-associated kinase.  |  Matsuoka, M., et al. 1994. Mol Cell Biol. 14: 7265-75. PMID: 7935441
7. Ordering S phase and M phase in the cell cycle.  |  Nurse, P. 1994. Cell. 79: 547-50. PMID: 7954820
8. G1 phase progression: cycling on cue.  |  Sherr, CJ. 1994. Cell. 79: 551-5. PMID: 7954821
9. Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age.  |  Hunter, T. and Pines, J. 1994. Cell. 79: 573-82. PMID: 7954824
10. Regulation of cyclin D-dependent kinase 4 (cdk4) by cdk4-activating kinase.  |  Kato, JY., et al. 1994. Mol Cell Biol. 14: 2713-21. PMID: 8139570
11. Molecular cloning of CDK7-associated human MAT1, a cyclin-dependent kinase-activating kinase (CAK) assembly factor.  |  Yee, A., et al. 1995. Cancer Res. 55: 6058-62. PMID: 8521393
12. In vitro assembly of a functional human CDK7-cyclin H complex requires MAT1, a novel 36 kDa RING finger protein.  |  Tassan, JP., et al. 1995. EMBO J. 14: 5608-17. PMID: 8521818

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mat1 Antibody (F-6) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Mat1 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mat1 Antibody (F-6) Specifications

Alternate Product Name: Mat1

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-309

Epitope Target: Mat1

Epitope Species: human

Product Reactivity/Detection: Mat1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Mat1 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine