MAT Iα/IIα Antibody (A-10): sc-166183
- MAT Iα/IIα Antibody (A-10) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of MAT IIα of human origin
- recommended for detection of MAT Iα and MAT IIα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAT Iα/IIα Antibody (A-10) for WB and IHC(P) applications. These reagents are now offered in bundles with MAT Iα/IIα Antibody (A-10) (see ordering information below).
MAT Iα/IIα Antibody (A-10) is a mouse monoclonal IgG1 antibody that detects Methionine adenosyltransferase (MAT) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MAT plays a crucial role in methionine metabolism by catalyzing S-adenosylmethionine (AdoMet) formation, essential for numerous biological processes, including methylation reactions that regulate gene expression and protein function. AdoMet′s importance extends to polyamine and phospholipid synthesis, vital for cell growth and membrane integrity. MAT Iα/IIα (A-10) antibody enables researchers to study MAT′s expression patterns, which when dysregulated can lead to liver diseases such as cirrhosis and hepatocellular carcinoma. Anti-MAT Iα/IIα antibody (A-10) serves as an invaluable tool for investigating MAT′s function and potential as a therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MAT Iα/IIα Antibody (A-10) References:
- Expression and functional interaction of the catalytic and regulatory subunits of human methionine adenosyltransferase in mammalian cells. | Halim, AB., et al. 1999. J Biol Chem. 274: 29720-5. PMID: 10514445
- Cloning, expression, and functional characterization of the beta regulatory subunit of human methionine adenosyltransferase (MAT II). | LeGros, HL., et al. 2000. J Biol Chem. 275: 2359-66. PMID: 10644686
- Small molecules, big effects: a role for chromatin-localized metabolite biosynthesis in gene regulation. | Gibson, BA. and Kraus, WL. 2011. Mol Cell. 41: 497-9. PMID: 21362545
- Methionine adenosyltransferase II serves as a transcriptional corepressor of Maf oncoprotein. | Katoh, Y., et al. 2011. Mol Cell. 41: 554-66. PMID: 21362551
- Metabolic aspects of epigenome: coupling of S-adenosylmethionine synthesis and gene regulation on chromatin by SAMIT module. | Igarashi, K. and Katoh, Y. 2013. Subcell Biochem. 61: 105-18. PMID: 23150248
- Methionine adenosyltransferase II-dependent histone H3K9 methylation at the COX-2 gene locus. | Kera, Y., et al. 2013. J Biol Chem. 288: 13592-601. PMID: 23539621
- MAT2A mutations predispose individuals to thoracic aortic aneurysms. | Guo, DC., et al. 2015. Am J Hum Genet. 96: 170-7. PMID: 25557781
- Acetylation of MAT IIα represses tumour cell growth and is decreased in human hepatocellular cancer. | Yang, HB., et al. 2015. Nat Commun. 6: 6973. PMID: 25925782
- Cullin 3 targets methionine adenosyltransferase IIα for ubiquitylation-mediated degradation and regulates colorectal cancer cell proliferation. | Wang, J., et al. 2016. FEBS J. 283: 2390-402. PMID: 27213918
- Dietary folate drives methionine metabolism to promote cancer development by stabilizing MAT IIA. | Li, JT., et al. 2022. Signal Transduct Target Ther. 7: 192. PMID: 35729157
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAT Iα/IIα Antibody (A-10) | sc-166183 | 200 µg/ml | $322.00 | |||
MAT Iα/IIα Antibody (): m-IgG Fc BP-HRP Bundle | sc-537451 | 200 µg Ab; 10 µg BP | $361.00 | |||
MAT Iα/IIα Antibody (): m-IgGκ BP-HRP Bundle | sc-534723 | 200 µg Ab; 40 µg BP | $361.00 | |||
MAT Iα/IIα Antibody (): m-IgG1 BP-HRP Bundle | sc-545189 | 200 µg Ab; 20 µg BP | $361.00 |