MASTL Antibody (235CT7.8.2.3): sc-517343

MASTL Antibody (235CT7.8.2.3) is a mouse monoclonal IgG1 kappa light chain antibody that detects MASTL protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-MASTL antibody (235CT7.8.2.3) is available as a non-conjugated format. MASTL, or microtubule associated serine/threonine kinase-like, is a crucial 879 amino acid serine/threonine kinase that plays a significant role in regulating the entry into and maintenance of mitosis, making MASTL essential for proper cell division and proliferation. MASTL is localized in the cytoplasm, nucleus, and cytoskeleton, which is important for coordinating the mitotic process. MASTL is a member of the AGC Ser/Thr protein kinase family and the protein kinase superfamily, and undergoes alternative splicing to produce three distinct isoforms. Additionally, MASTL is post-translationally phosphorylated by Cdc2 p34, a modification vital for activity and regulation during the cell cycle. The gene encoding MASTL is located on human chromosome 10, which harbors approximately 4.5% of total cellular DNA and encodes nearly 1,200 genes. Notably, defects in genes mapping to this chromosome have been linked to various genetic disorders, including Charcot-Marie-Tooth disease and multiple endocrine neoplasia type 2, highlighting MASTL′s importance in cellular function and potential implications in human health.

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MASTL Antibody (235CT7.8.2.3) References:

1. Patterns of somatic mutation in human cancer genomes.  |  Greenman, C., et al. 2007. Nature. 446: 153-8. PMID: 17344846
2. Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.  |  Cantin, GT., et al. 2008. J Proteome Res. 7: 1346-51. PMID: 18220336
3. Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.  |  Daub, H., et al. 2008. Mol Cell. 31: 438-48. PMID: 18691976
4. Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis.  |  Wang, B., et al. 2008. Anal Chem. 80: 9526-33. PMID: 19007248
5. Greatwall maintains mitosis through regulation of PP2A.  |  Vigneron, S., et al. 2009. EMBO J. 28: 2786-93. PMID: 19680222
6. The M phase kinase Greatwall (Gwl) promotes inactivation of PP2A/B55delta, a phosphatase directed against CDK phosphosites.  |  Castilho, PV., et al. 2009. Mol Biol Cell. 20: 4777-89. PMID: 19793917
7. MASTL is the human orthologue of Greatwall kinase that facilitates mitotic entry, anaphase and cytokinesis.  |  Voets, E. and Wolthuis, RM. 2010. Cell Cycle. 9: 3591-601. PMID: 20818157
8. The substrate of Greatwall kinase, Arpp19, controls mitosis by inhibiting protein phosphatase 2A.  |  Gharbi-Ayachi, A., et al. 2010. Science. 330: 1673-7. PMID: 21164014