Mast Cell Tryptase Antibody (H-9): sc-271095

Mast Cell Tryptase Antibody (H-9) is a mouse monoclonal IgG2a antibody that detects mast cell tryptase (MCT) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MCT antibody (H-9) specifically targets mast cell tryptase, a crucial serine protease that plays a significant role in inflammatory response and tissue remodeling. MCT monoclonal antibody (H-9) recognizes a protein primarily located in mast cells, which are essential components of the immune system, as they release mediators that influence vascular permeability and recruit other immune cells to sites of injury or infection. The presence of mast cell tryptase in tissues can indicate mast cell activation and is often associated with allergic reactions and asthma, making MCT (H-9) antibody a valuable tool for studying various inflammatory conditions. Additionally, mast cell tryptase exists as a tetrameric enzyme, which is stabilized by heparin, enhancing its enzymatic activity and allowing it to effectively participate in the degradation of extracellular matrix components. Understanding the localization and function of mast cell tryptase is vital for elucidating its role in both normal physiology and pathological conditions, thereby providing insights into potential therapeutic targets for allergic and inflammatory diseases.

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Mast Cell Tryptase Antibody (H-9) References:

1. Characterization of mast-cell tryptase-expressing peripheral blood cells as basophils.  |  Foster, B., et al. 2002. J Allergy Clin Immunol. 109: 287-93. PMID: 11842299
2. Mast cell tryptase degrades HDL and blocks its function as an acceptor of cellular cholesterol.  |  Lee, M., et al. 2002. Arterioscler Thromb Vasc Biol. 22: 2086-91. PMID: 12482839
3. Mast cell tryptase stimulates DLD-1 carcinoma through prostaglandin- and MAP kinase-dependent manners.  |  Yoshii, M., et al. 2005. J Pharmacol Sci. 98: 450-8. PMID: 16093613
4. Mast cell tryptase and photoaging: possible involvement in the degradation of extra cellular matrix and basement membrane proteins.  |  Iddamalgoda, A., et al. 2008. Arch Dermatol Res. 300 Suppl 1: S69-76. PMID: 17968569
5. Immunohistochemical expression of mast cell tryptase in giant cell fibroma and inflammatory fibrous hyperplasia of the oral mucosa.  |  Santos, PP., et al. 2011. Arch Oral Biol. 56: 231-7. PMID: 21035108
6. Mast cell tryptase stimulates myoblast proliferation; a mechanism relying on protease-activated receptor-2 and cyclooxygenase-2.  |  Duchesne, E., et al. 2011. BMC Musculoskelet Disord. 12: 235. PMID: 21999702
7. A relation between TGF-β and mast cell tryptase in experimental emphysema models.  |  Mortaz, E., et al. 2012. Biochim Biophys Acta. 1822: 1154-60. PMID: 22481124
8. Mast cell tryptase induces microglia activation via protease-activated receptor 2 signaling.  |  Zhang, S., et al. 2012. Cell Physiol Biochem. 29: 931-40. PMID: 22613992
9. Computational modeling of mast cell tryptase family informs selective inhibitor development.  |  Ma, Y., et al. 2024. iScience. 27: 110739. PMID: 39280611
10. Human mast cell tryptase: a stimulus of microvascular leakage and mast cell activation.  |  He, S. and Walls, AF. 1997. Eur J Pharmacol. 328: 89-97. PMID: 9203574

The preferred Mast Cell Tryptase antibody is Mast Cell Tryptase Antibody (G3): sc-33676.