Date published: 2025-9-5
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Mast Cell Tryptase Antibody (G3) - Western Blotting - Image 355306
肥大细胞胰蛋白酶 (G3): sc-33676. U-937 (A) 和 A-673 (B) 全细胞裂解液中肥大细胞胰蛋白酶表达的 Western 印迹分析.
肥大细胞胰蛋白酶 (G3): sc-33676. U-937 (A) 和 A-673 (B) 全细胞裂解液中肥大细胞胰蛋白酶表达的 Western 印迹分析.

Mast Cell Tryptase 抗体 (G3): sc-33676

5.0(7)
寫評論提問

说明书
  • Mast Cell Tryptase 抗体 G3 是小鼠单克隆 IgG1 κ，Mast Cell Tryptase抗体, 在17篇文献中引用，规格为200 µg/ml
  • 针对human从肺肥大细胞中纯化的胰蛋白酶
  • Mast Cell Tryptase 抗体 (G3) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 Mast Cell Tryptase (also designated MMCP-6)
  • 抗Mast Cell Tryptase抗体（G3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mast Cell Tryptase (G3): sc-33676的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Mast Cell Tryptase 抗体 (G3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Mast Cell Tryptase 抗体 (G3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

肥大细胞蛋白酶抗体（G3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测人源肥大细胞蛋白酶蛋白。肥大细胞蛋白酶（G3）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。肥大细胞蛋白酶在免疫反应中起着至关重要的作用，主要通过调节炎症和组织重塑。肥大细胞是位于结缔组织中的特殊免疫细胞，可分泌这种蛋白，参与过敏反应和抵御病原体。肥大细胞蛋白酶主要位于肥大细胞的分泌颗粒中，以非活性形式储存，直到细胞激活触发释放。这种特殊的定位使得组织蛋白酶能够在炎症部位快速释放，从而调节血管通透性和免疫细胞募集。由肝素稳定的多聚体结构可增强酶的活性，并有效切割各种底物，包括细胞外基质成分和信号分子，从而影响整体炎症反应。了解肥大细胞组织蛋白酶的功能和定位对于阐明其在哮喘、过敏性反应和其他过敏性疾病等各种病理条件下的作用仍然至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mast Cell Tryptase 抗体 (G3) 参考文献:

  1. 肥大细胞的多种作用  |  Gurish, MF. and Austen, KF. 2001. J Exp Med. 194: F1-5. PMID: 11435478
  2. 三种小鼠肥大细胞特异性丝氨酸蛋白酶 MMCP-1, MMCP-4 和 MMCP-5 的克隆与结构分析。  |  Huang, RY., et al. 1991. Eur J Immunol. 21: 1611-21. PMID: 2060576
  3. 人类肥大细胞糜蛋白酶基因的结构, 染色体分配和推导的氨基酸序列。  |  Caughey, GH., et al. 1991. J Biol Chem. 266: 12956-63. PMID: 2071582
  4. 生物学和医学中的人类肥大细胞胰蛋白酶。  |  Vitte, J. 2015. Mol Immunol. 63: 18-24. PMID: 24793463
  5. 肥大细胞胰蛋白酶--心血管疾病的标志物和制造者。  |  Mohajeri, M., et al. 2019. Pharmacol Ther. 199: 91-110. PMID: 30877022
  6. 肥大细胞胰蛋白酶促进炎症性肠病诱发的肠纤维化  |  Liu, B., et al. 2021. Inflamm Bowel Dis. 27: 242-255. PMID: 32507895
  7. 乳腺细胞色氨酸酶的释放有助于淋巴管瘤病的病情发展  |  Babaei-Jadidi, R., et al. 2021. Am J Respir Crit Care Med. 204: 431-444. PMID: 33882264
  8. 肥大细胞色氨酸酶通过促进人支气管上皮细胞的迁移来增强伤口愈合。  |  Mogren, S., et al. 2021. Cell Adh Migr. 15: 202-214. PMID: 34308764
  9. 肥大细胞胰蛋白酶能促进中性粒细胞胞外捕获器的形成  |  Pejler, G., et al. 2022. J Innate Immun. 14: 433-446. PMID: 34937018
  10. 肥大细胞蛋白酶通过诱导人类肺泡和支气管上皮细胞的抗凋亡和细胞生长特性来促进气道重塑。  |  Berlin, F., et al. 2023. Cells. 12: PMID: 37408273
  11. TLR7 通过肥大细胞胰蛋白酶的活性促进烟雾诱发的实验性肺损伤。  |  Liu, G., et al. 2023. Nat Commun. 14: 7349. PMID: 37963864
  12. 人类肥大细胞糜蛋白酶基因（CMA1）：与糜蛋白酶 G/酶基因簇的映射关系及系限制性表达。  |  Caughey, GH., et al. 1993. Genomics. 15: 614-20. PMID: 8468056

订购信息

产品名称产品编号规格价格数量收藏夹

Mast Cell Tryptase 抗体 (G3)

sc-33676
200 µg/ml
$316.00

Mast Cell Tryptase (G3)Korey): m-IgG Fc BP-HRP 套装

sc-526702
200 µg Ab; 10 µg BP
$354.00

Mast Cell Tryptase (G3)Korey): m-IgG1 BP-HRP 套装

sc-532075
200 µg Ab; 20 µg BP
$354.00

Mast Cell Tryptase 抗体 (G3) AC

sc-33676 AC
500 µg/ml, 25% agarose
$416.00

Mast Cell Tryptase 抗体 (G3) HRP

sc-33676 HRP
200 µg/ml
$316.00

Mast Cell Tryptase 抗体 (G3) FITC

sc-33676 FITC
200 µg/ml
$330.00

Mast Cell Tryptase 抗体 (G3) PE

sc-33676 PE
200 µg/ml
$343.00

Mast Cell Tryptase 抗体 (G3) Alexa Fluor® 488

sc-33676 AF488
200 µg/ml
$357.00

Mast Cell Tryptase 抗体 (G3) Alexa Fluor® 546

sc-33676 AF546
200 µg/ml
$357.00

Mast Cell Tryptase 抗体 (G3) Alexa Fluor® 594

sc-33676 AF594
200 µg/ml
$357.00

Mast Cell Tryptase 抗体 (G3) Alexa Fluor® 647

sc-33676 AF647
200 µg/ml
$357.00

Mast Cell Tryptase 抗体 (G3) Alexa Fluor® 680

sc-33676 AF680
200 µg/ml
$357.00

Mast Cell Tryptase 抗体 (G3) Alexa Fluor® 790

sc-33676 AF790
200 µg/ml
$357.00

What is the optimal concentration for flow testing? what is the Isotype? Cat #:sc-33676; Batch #:K0519

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-10

Can Mast Cell Tryptase (G3): sc-33676 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Mast Cell Tryptase (G3): sc-33676 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_29, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_33676, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 120ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from G3 Works Great for IHCAs the title suggests, I used this antibody very successfully for IHC of Human Mast Cells. I will be using this antibody in the future.
Date published: 2017-10-20
Rated 5 out of 5 by from Beautiful Immunohistochemistry staining!Very clean and specific, this antibody is a winner for IHC. It's also great for WB and IF, so it's a strong antibody all-around. Great choice!
Date published: 2017-01-24
Rated 5 out of 5 by from Strong band in western blot analysis of humanStrong band in western blot analysis of human recombinant Tryptase. -SCBT QC
Date published: 2015-06-23
Rated 5 out of 5 by from Robust band in western blot analysis of MastRobust band in western blot analysis of Mast Cell Tryptase expression in human fetal skin tissue extract. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Published IF data using human nasal biopsy samplesPublished IF data using human nasal biopsy samples. -SCBT Publication Review
Date published: 2015-04-13
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human stomach tissue. -SCBT QC
Date published: 2015-03-31
Rated 5 out of 5 by from Works great for western blot with tranfectedWorks great for western blot with tranfected lysates. -SCBT QC
Date published: 2015-01-28
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_33676, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 13ms
  • REVIEWS, PRODUCT
Mast Cell Tryptase 抗体 (G3) is rated 5.0 out of 5 by 7.
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_33676, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 119ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。