MASP-1/3 CRISPR/Cas9 KO Plasmid (m): sc-421564
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MASP-1/3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MASP-1/3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MASP-1/3 Antibody (F-12): sc-166815
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MASP-1/3 CRISPR/Cas9 KO Plasmid (m) | sc-421564 | 20 µg | $397.00 |
Overview
Masp1 encodes mannose-binding lectin–associated serine proteases MASP-1 and MASP-3, secreted enzymes that participate in innate immunity by regulating the lectin pathway of complement. Through cleavage of complement components and coordination with pattern-recognition molecules such as MBL and ficolins, MASP-1/3 influences opsonization, inflammatory signaling, and crosstalk between complement and coagulation-related processes. MASP-3 is additionally linked to alternative pathway control via activation of factor D, positioning Masp1 at a key intersection of complement amplification. Dysregulated complement activation and altered MASP-1/3 activity have been associated with inflammatory and autoimmune phenotypes and contribute to disease-relevant tissue injury mechanisms in multiple organ systems.
MASP-1/3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Masp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Masp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Masp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MASP-1/3 protein expression.
This CRISPR knockout system enables efficient generation of Masp1-deficient cell models for investigation of MASP-1/3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Masp1 exon(s) critical for MASP-1/3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Masp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MASP-1/3 CRISPR/Cas9 KO Plasmid (m) and MASP-1/3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Masp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MASP-1/3 HDR Plasmid (m) and MASP-1/3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Masp1 homology arms to support homology-directed repair at defined Masp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.