MARK3 Antibody (G-7): sc-376115

MARK3 Antibody (G-7) is a mouse monoclonal IgG2a antibody that detects MARK3 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MARK3, also known as MAP/microtubule affinity-regulating kinase 3, C-TAK1, EMK-2, protein kinase STK10, or serine/threonine-protein kinase p78, is a 753 amino acid peripheral membrane protein that plays a crucial role in cell cycle regulation by phosphorylating Cdc25C on Serine 216, thereby acting as a negative regulator of mitosis. This regulation is vital for maintaining proper cell division and preventing uncontrolled proliferation, which can lead to tumorigenesis. MARK3 is expressed in various human tissues and cell lines and exists in six alternatively spliced isoforms, indicating diverse functional roles. The gene encoding MARK3 is located on human chromosome 14q32.3 and mouse chromosome 12 F1, highlighting evolutionary conservation across species. MARK3′s ability to mediate the binding of 14-3-3 proteins through kinase activity further underscores importance in cellular signaling pathways, making anti-MARK3 antibody (G-7) an essential tool for researchers studying cell cycle dynamics and related diseases.

The preferred MARK3 antibody is MARK3 Antibody (G-11): sc-365461.